Badwelan Mohammed, Alkindi Mohammed, Alghamdi Osama, Ahmed Abeer, Ramalingam Sundar, Alrahlah Ali
Department of Oral and Maxillofacial Surgery, College of Dentistry, King Saud University, Riyadh 11545, Saudi Arabia.
Department of Periodontics and Community Dentistry, College of Dentistry, King Saud University, Riyadh 11545, Saudi Arabia.
Materials (Basel). 2021 May 3;14(9):2384. doi: 10.3390/ma14092384.
Bone regeneration using beta-tricalcium phosphate (β-TCP) can be practiced using a biocomposite scaffold. Poly(ethylene-co-vinylalcohol)/poly(δ-valerolactone)/β-tricalcium phosphate (PEVAV/β-TCP) composite scaffolds showed promising in vitro results. This study evaluated the bone regenerative potential of PEVAV/β-TCP biocomposite scaffolds in standardized calvarial defects in a rat model over 4 and 10 weeks. Bilateral calvarial defects (5 mm in diameter and about 1.5 mm thick, equivalent to the thickness of the calvaria) were created in 40 male Wistar albino rats. The defects were grafted with either commercially available β-TCP (positive control), PEVAV/β-TCP 70, or PEVAV/β-TCP 50, or left empty (negative control), depending on the group to which the animal was randomly assigned, to be covered before flap closure with resorbable collagen membrane (RCM). At 4 and 10 weeks post-surgery, the collected rat calvaria were evaluated using micro computed tomography (micro-CT) analysis, to assess the newly formed bone volume (NFBV), newly formed bone mineral density (NFBMD), and remaining graft volume (RGV). The results showed that calvarial defects grafted with the PEVAV/β-TCP biocomposite exhibited higher NFBV than did control defects, both at 4 and 10 weeks post-surgery. Furthermore, calvarial defects grafted with PEVAV/β-TCP 70 showed the highest NFBV among all grafting conditions, with a statistically significant difference recorded at 10 weeks post-surgery. The PEVAV/β-TCP composite scaffold showed potentiality for the regeneration of critical-sized calvarial bone defects in a rat model.
使用β-磷酸三钙（β-TCP）进行骨再生可通过生物复合支架来实现。聚（乙烯-共-乙烯醇）/聚（δ-戊内酯）/β-磷酸三钙（PEVAV/β-TCP）复合支架在体外实验中显示出良好的结果。本研究评估了PEVAV/β-TCP生物复合支架在大鼠模型标准化颅骨缺损中4周和10周后的骨再生潜力。在40只雄性Wistar白化大鼠中制造双侧颅骨缺损（直径5mm，厚约1.5mm，等同于颅骨厚度）。根据动物随机分组情况，缺损处分别植入市售β-TCP（阳性对照）、PEVAV/β-TCP 70或PEVAV/β-TCP 50，或留空（阴性对照），在皮瓣闭合前用可吸收胶原膜（RCM）覆盖。术后4周和10周，使用微型计算机断层扫描（micro-CT）分析对收集的大鼠颅骨进行评估，以评估新形成的骨体积（NFBV）、新形成的骨矿物质密度（NFBMD）和剩余移植物体积（RGV）。结果显示，在术后4周和10周，植入PEVAV/β-TCP生物复合材料的颅骨缺损处的NFBV均高于对照缺损处。此外，在所有植入条件下，植入PEVAV/β-TCP 70的颅骨缺损处显示出最高的NFBV，术后10周有统计学显著差异。PEVAV/β-TCP复合支架在大鼠模型中对临界尺寸颅骨骨缺损的再生显示出潜力。
