Shaheen Marwa Y, Basudan Amani M, Niazy Abdurahman A, van den Beucken Jeroen J J P, Jansen John A, Alghamdi Hamdan S
Department of Periodontics and Community Dentistry, College of Dentistry, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
Department of Oral Medicine and Diagnostic Sciences, College of Dentistry, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
Materials (Basel). 2021 Jan 5;14(1):222. doi: 10.3390/ma14010222.
We evaluated the effect of osteoporotic induction after eight weeks of initial healing of bone defects grafted with a xenograft material in a rat model. Bone defects were created in the femoral condyles of 16 female Wistar rats (one defect per rat). The defects were filled with bovine bone (Inter-Oss) granules. After eight weeks of bone healing, rats were randomly ovariectomized (OVX) or sham-operated (SHAM). At 14 weeks of bone healing, all animals were euthanized. Bone specimens were harvested and processed for histological and histomorphometric analyses to assess new bone formation (N-BF%), remaining bone graft (RBG%) and trabecular bone space (Tb.Sp%) within the defect area. After 14 weeks of bone healing, histological evaluation revealed a significant alteration in trabecular bone in OVX rats compared to SHAM rats. There was lower N-BF% in OVX rats (22.5% ± 3.0%) compared to SHAM rats (37.7% ± 7.9%; < 0.05). Additionally, the RBG% was significantly lower in OVX (23.7% ± 5.8%) compared to SHAM (34.8% ± 9.6%; < 0.05) rats. Finally, the Tb.Sp% was higher in OVX (53.8% ± 7.7%) compared to SHAM (27.5% ± 14.3%; < 0.05) rats. In conclusion, within the limitations of this study, inducing an osteoporotic condition in a rat model negatively influenced bone regeneration in the created bone defect and grafted with a xenograft material.
我们在大鼠模型中评估了用异种移植材料移植骨缺损初始愈合八周后诱导骨质疏松的效果。在16只雌性Wistar大鼠的股骨髁上制造骨缺损（每只大鼠一个缺损）。缺损处填充牛骨（Inter - Oss）颗粒。骨愈合八周后，将大鼠随机进行卵巢切除（OVX）或假手术（SHAM）。在骨愈合14周时，对所有动物实施安乐死。采集骨标本并进行组织学和组织形态计量学分析，以评估缺损区域内的新骨形成（N - BF%）、剩余骨移植量（RBG%）和小梁骨间隙（Tb.Sp%）。骨愈合14周后，组织学评估显示，与SHAM大鼠相比，OVX大鼠的小梁骨有显著改变。与SHAM大鼠（37.7% ± 7.9%；P < 0.05）相比，OVX大鼠的N - BF%较低（22.5% ± 3.0%）。此外，与SHAM大鼠（34.8% ± 9.6%；P < 0.05）相比，OVX大鼠的RBG%显著较低（23.7% ± 5.8%）。最后，与SHAM大鼠（27.5% ± 14.3%；P < 0.05）相比，OVX大鼠的Tb.Sp%较高（53.8% ± 7.7%）。总之，在本研究的局限性范围内，在大鼠模型中诱导骨质疏松状态对所制造的骨缺损并用异种移植材料移植后的骨再生产生了负面影响。
