Shi Jinping, Wang Xueying, Song Yali, Liu Ting, Cheng Shuru, Zhang Quanwei
College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China.
Animals (Basel). 2021 May 21;11(6):1492. doi: 10.3390/ani11061492.
Crossbreeding can improve production performance and meat quality in sheep. The objective of this study was to look for genes related to sheep growth, development, and muscle. In this study, Dorper (DP) × Small Tailed Han (STH) sheep and Mongolia (MG) × Small-tailed Han (STH) sheep were used to estimate the productive performance and meat quality in a crossbreed. Subsequently, transcriptome analysis and bioinformatic analysis were performed on the muscles of DP × STH and MG × STH sheep to identify differentially expressed genes (DEGs) related to growth, development, and meat quality. The presence of DEGs was confirmed by real-time PCR (qPCR). Productive performance and meat quality of the DP × STH sheep were better than the MG × STH sheep. Compared to DP × STH, a total of 1445 DEGs were identified in MG × STH sheep (1026 DEG were up-regulated and 419 DEG were down-regulated). Of these, 38 DEGs were related to growth, 161 to development, and 43 to muscle. In addition, 13 co-expressed genes (, , , , , , , , , , , , and ) were identified. The expression of DEGs was consistent with the comparative transcriptome analysis. This work provides genetics resources for future research on muscle development in sheep.
杂交育种可以提高绵羊的生产性能和肉质。本研究的目的是寻找与绵羊生长、发育和肌肉相关的基因。在本研究中，采用杜泊（DP）×小尾寒羊（STH）和蒙古羊（MG）×小尾寒羊（STH）来评估杂交后代的生产性能和肉质。随后，对DP×STH和MG×STH绵羊的肌肉进行转录组分析和生物信息学分析，以鉴定与生长、发育和肉质相关的差异表达基因（DEGs）。通过实时定量PCR（qPCR）确认了DEGs的存在。DP×STH绵羊的生产性能和肉质优于MG×STH绵羊。与DP×STH相比，MG×STH绵羊中共鉴定出1445个DEGs（1026个上调，419个下调）。其中，38个DEGs与生长相关，161个与发育相关，43个与肌肉相关。此外，还鉴定出13个共表达基因（，，，，，，，，，，，，和）。DEGs的表达与比较转录组分析结果一致。本研究为未来绵羊肌肉发育的研究提供了遗传资源。
