College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China.
Genes (Basel). 2020 Feb 10;11(2):183. doi: 10.3390/genes11020183.
Crossbred sheep have many prominent traits, such as excellent production performance and high-quality meat, when compared to local sheep breeds. However, the genetic molecular markers related to these characteristics remain unclear. The crossbred MG × STH (small-tailed Han sheep (STH) × Mongolian sheep (MG)) breed and the STH breed were selected to measure production performance and meat quality. We used 14 indexes of production performance and meat quality, which in the MG × STH population showed significant differences compared to the STH breed. Subsequently, the from the two sheep were subjected to comparative transcriptomic analyses to identify differentially expressed genes (DEGs) related to production performance and meat quality. A total of 874 DEGs were identified between the two sheep groups. A total of 110 unique DEGs related to sheep production performance and meat quality were selected as the candidate DEGs. We found 6 production-performance-related and 30 meat-quality-related DEGs through a correlation analysis, including , , and . The expression levels of 11 DEGs were validated by real-time PCR, and the results were in accordance with the results of the comparative transcriptomic and correlation analyses. These results will assist in understanding sheep heterosis and molecular marker-assisted selection.
与本地绵羊品种相比，杂交羊具有许多突出的特性，如优良的生产性能和优质的肉。然而，与这些特性相关的遗传分子标记仍不清楚。选择杂交 MG×STH（小尾寒羊（STH）×蒙古羊（MG））品种和 STH 品种来衡量生产性能和肉质。我们使用了 14 项生产性能和肉质指标，MG×STH 群体的这些指标与 STH 品种相比有显著差异。随后，对来自这两个绵羊品种的进行了比较转录组分析，以鉴定与生产性能和肉质相关的差异表达基因（DEGs）。在这两个绵羊群体之间共鉴定出 874 个 DEGs。共选择了 110 个与绵羊生产性能和肉质相关的独特 DEGs 作为候选 DEGs。通过相关性分析，我们发现了 6 个与生产性能相关和 30 个与肉质相关的 DEGs，包括、、和。通过实时 PCR 验证了 11 个 DEG 的表达水平，结果与比较转录组和相关性分析的结果一致。这些结果将有助于理解绵羊杂种优势和分子标记辅助选择。
