Cheng Shuru, Wang Xueyin, Wang Qi, Yang Lei, Shi Jinping, Zhang Quanwei
College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China.
Genomics. 2020 Sep;112(5):3322-3330. doi: 10.1016/j.ygeno.2020.06.011. Epub 2020 Jun 10.
From a genetic perspective, the advantages of crossbreeding in sheep are unclear. In the present study, a comparative transcriptomic analysis was performed using Longissimus dorsi tissues from two sheep groups in order to identify differentially expressed genes (DEGs) related to growth, development and meat quality. Compared to Small Tail Han sheep, a total of 874 DEGs were identified in the crossbred sheep. Among these DEGs, 30, 116 and 32 DEGs were related to growth, development and meat quality, respectively. Seven DEGs highlighted by functional analysis as playing crucial roles in growth, development and meat quality were validated by the gene-act-network and co-expression-network. The expression levels of DEG mRNAs and proteins were further confirmed using RT-qPCR and western blot analyses. The results were consistent with the comparative transcriptome data. The data from this transcriptomic analysis will help to understand genetic heterosis and molecular-assisted breeding in sheep.
从遗传学角度来看，绵羊杂交育种的优势尚不清楚。在本研究中，为了鉴定与生长、发育和肉质相关的差异表达基因（DEGs），对两个绵羊群体的背最长肌组织进行了比较转录组分析。与小尾寒羊相比，在杂交绵羊中总共鉴定出874个DEGs。在这些DEGs中，分别有30、116和32个DEGs与生长、发育和肉质相关。通过基因作用网络和共表达网络验证了7个经功能分析突出显示在生长、发育和肉质中起关键作用的DEGs。使用RT-qPCR和蛋白质印迹分析进一步证实了DEG mRNA和蛋白质的表达水平。结果与比较转录组数据一致。该转录组分析的数据将有助于理解绵羊的遗传杂种优势和分子辅助育种。
