Laboratory of Food Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771 Athens, Greece.
Laboratory of Analytical Chemistry, Department of Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771 Athens, Greece.
Molecules. 2021 May 11;26(10):2837. doi: 10.3390/molecules26102837.
Wine metabolomics constitutes a powerful discipline towards wine authenticity assessment through the simultaneous exploration of multiple classes of compounds in the wine matrix. Over the last decades, wines from autochthonous Greek grape varieties have become increasingly popular among wine connoisseurs, attracting great interest for their authentication and chemical characterization. In this work, 46 red wine samples from and grape varieties were collected from wineries in two important winemaking regions of Greece during two consecutive vintages and analyzed using ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QToF-MS). A targeted metabolomics methodology was developed, including the determination and quantification of 28 phenolic compounds from different classes (hydroxycinnamic acids, hydroxybenzoic acids, stilbenes and flavonoids). Moreover, 86 compounds were detected and tentatively identified via a robust suspect screening workflow using an in-house database of 420 wine related compounds. Supervised chemometric techniques were employed to build an accurate and robust model to discriminate between two varieties.
葡萄酒代谢组学通过同时探索葡萄酒基质中的多类化合物，构成了评估葡萄酒真实性的强大学科。在过去的几十年中，来自希腊本土葡萄品种的葡萄酒在葡萄酒鉴赏家中越来越受欢迎，因其真实性和化学特征而引起了极大的关注。在这项工作中，从两个连续的年份在希腊两个重要的酿酒区的酒庄中收集了来自 和 葡萄品种的 46 个红葡萄酒样本，并使用超高效液相色谱-四极杆飞行时间质谱（UHPLC-QToF-MS）进行了分析。开发了一种靶向代谢组学方法，包括测定和定量来自不同类别的 28 种酚类化合物（羟基肉桂酸、羟基苯甲酸、芪和类黄酮）。此外，通过使用内部 420 种与葡萄酒相关的化合物数据库的强大可疑筛选工作流程，检测并初步鉴定了 86 种化合物。采用有监督的化学计量学技术来建立一个准确和稳健的模型，以区分两个品种。
