Evdokias Gregory, Semper Cameron, Mora-Ochomogo Montserrat, Di Falco Marcos, Nguyen Thi Truc Minh, Savchenko Alexei, Tsang Adrian, Benoit-Gelber Isabelle
Centre for Structural and Functional Genomics, Department of Biology, Concordia University, 7141 Rue Sherbrooke Ouest, Montréal, QC H4B 1R6, Canada.
Department of Microbiology, Immunology and Infectious Disease, University of Calgary, 3330 Hospital Drive, Calgary, AB T2N 4N1, Canada.
J Fungi (Basel). 2021 May 11;7(5):374. doi: 10.3390/jof7050374.
Previously, DNA microarrays analysis showed that, in co-culture with , a biosynthetic gene cluster anchored with a nonribosomal peptides synthetase of is downregulated. Based on phylogenetic and synteny analyses, we show here that this gene cluster, , comprises genes predicted to encode a nonribosomal peptides synthetase, a FAD-binding domain-containing protein, an uncharacterized protein, a transporter, a cytochrome P450 protein, a NAD(P)-binding domain-containing protein and a transcription factor. We overexpressed the in-cluster transcription factor gene . The overexpression strain, NRRL3_00042, displays reduced growth rate and production of a yellow pigment, which by mass spectrometric analysis corresponds to two compounds with masses of 409.1384 and 425.1331. We deleted the gene encoding the NRRL3_00036 nonribosomal peptides synthetase in the NRRL3_00042 strain. The resulting strain reverted to the wild-type phenotype. These results suggest that the biosynthetic gene cluster anchored by the nonribosomal peptides synthetase gene is regulated by the in-cluster transcriptional regulator gene , and that it is involved in the production of two previously uncharacterized compounds.
此前，DNA微阵列分析表明，在与……共培养时，一个由……的非核糖体肽合成酶锚定的生物合成基因簇被下调。基于系统发育和共线性分析，我们在此表明，这个基因簇……包含预测编码非核糖体肽合成酶、含FAD结合结构域的蛋白质、未表征的蛋白质、转运蛋白、细胞色素P450蛋白、含NAD(P)结合结构域的蛋白质和转录因子的基因。我们过表达了簇内转录因子基因……。过表达菌株NRRL3_00042显示出生长速率降低和产生一种黄色色素，通过质谱分析，该色素对应于两种质量分别为409.1384和425.1331的化合物。我们在NRRL3_00042菌株中删除了编码NRRL3_00036非核糖体肽合成酶的基因。所得菌株恢复为野生型表型。这些结果表明，由……非核糖体肽合成酶基因锚定的生物合成基因簇受簇内转录调节基因……调控，并且它参与了两种先前未表征化合物的产生。
