Fowler Emily Celeste, Poudel Prakash, White Brandon, St-Pierre Benoit, Brown Michael
Department of Animal Science, South Dakota State University, Brookings, SD 57007, USA.
Department of Natural Resource Management, South Dakota State University, Brookings, SD 57007, USA.
Microorganisms. 2021 May 11;9(5):1032. doi: 10.3390/microorganisms9051032.
The hybrid striped bass () is a carnivorous species and a major product of US aquaculture. To reduce costs and improve resource sustainability, traditional ingredients used in fish diets are becoming more broadly replaced by plant-based products; however, plant meals can be problematic for carnivorous fish. Bioprocessing has improved nutritional quality and allowed higher inclusions in fish diets, but these could potentially affect other systems such as the gut microbiome. In this context, the effects of bioprocessed soybean meal on the intestinal bacterial composition in hybrid striped bass were investigated. Using high-throughput sequencing of amplicons targeting the V1-V3 region of the 16S rRNA gene, no significant difference in bacterial composition was observed between fish fed a control diet, and fish fed a diet with the base bioprocessed soybean meal. The prominent Operational Taxonomic Unit (OTU) in these samples was predicted to be a novel species affiliated to . In contrast, the intestinal bacterial communities of fish fed bioprocessed soybean meal that had been further modified after fermentation exhibited lower alpha diversity ( < 0.05), as well as distinct and more varied composition patterns, with OTUs predicted to be strains of , , or being the most dominant. Together, these results suggest that compounds in bioprocessed soybean meal can affect intestinal bacterial communities in hybrid striped bass.
杂交条纹鲈（）是一种肉食性物种，也是美国水产养殖的主要产品。为了降低成本并提高资源可持续性，鱼类饲料中使用的传统成分正越来越多地被植物性产品所取代；然而，植物性饲料对肉食性鱼类可能存在问题。生物加工提高了营养质量，并允许在鱼类饲料中更高比例地使用，但这些可能会潜在地影响其他系统，如肠道微生物群。在此背景下，研究了生物加工豆粕对杂交条纹鲈肠道细菌组成的影响。通过对靶向16S rRNA基因V1 - V3区域的扩增子进行高通量测序，发现喂食对照饲料的鱼和喂食含基础生物加工豆粕饲料的鱼之间，细菌组成没有显著差异。这些样本中突出的操作分类单元（OTU）预计是一个隶属于的新物种。相比之下，喂食发酵后进一步改性的生物加工豆粕的鱼的肠道细菌群落，其α多样性较低（<0.05），并且组成模式独特且更多样化，预计最主要的OTU是、或的菌株。总之，这些结果表明生物加工豆粕中的化合物会影响杂交条纹鲈的肠道细菌群落。
