Zhang Hongyu, Wang Haibo, Hu Kun, Jiao Liting, Zhao Mingjun, Yang Xianle, Xia Lei
National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 210306, China.
Chinese Academy of Fishery Sciences, Beijing 100141, China.
Animals (Basel). 2019 Nov 20;9(12):1005. doi: 10.3390/ani9121005.
The purpose of this study is to investigate the effect of probiotics YYL3 (Lc) and YYL5 (Lp) on growth performance, innate immunity, disease resistance and intestinal microbiota of channel catfish. A total of 252 catfish (67.20 ± 1.46 g) were randomly divided into 3 groups which were fed with basal diet, Lc-added (3.0 × 10 cfu/g) or Lp-added (3.0 × 10 cfu/g) diets, respectively. After 4 weeks of feeding, Lc significantly enhanced the growth and feed utilization of channel catfish compared with the control group (CG). Following that, the catfish were challenged with an intraperitoneal injection of 200 μL of the pathogenic (2.0 × 10 cfu/mL), the relative percent survival of Lc and Lp were 38.28% and 12.76%, respectively. High-throughput sequencing indicated Lc and Lp reduced the alpha diversity of the intestinal microbiota in channel catfish. were overwhelming in the guts during probiotics treatment, but almost vanished away after 2 weeks post-cessation of probiotics administration. Compared to CG, Lc and Lp resulted in an increased abundance of and decreased amount of . Functional analysis revealed that Lc treatment upregulated the relative abundance of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways including lipid metabolism, metabolism of other amino acids, metabolism of terpenoids and polyketides, xenobiotics biodegradation and metabolism, and nucleotide metabolism. Combined, our data revealed that Lc, as a feed additive at 3.0 × 10 cfu/g, could promote the growth performance, disease resistance and dramatically change the composition of intestinal microbiota of channel catfish.
本研究旨在探讨益生菌YYL3（Lc）和YYL5（Lp）对斑点叉尾鮰生长性能、先天免疫、抗病能力及肠道微生物群的影响。总共252尾斑点叉尾鮰（67.20±1.46克）被随机分为3组，分别投喂基础饲料、添加Lc（3.0×10 cfu/g）或添加Lp（3.0×10 cfu/g）的饲料。投喂4周后，与对照组（CG）相比，Lc显著提高了斑点叉尾鮰的生长和饲料利用率。随后，给斑点叉尾鮰腹腔注射200μL致病性（2.0×10 cfu/mL）进行攻毒，Lc和Lp的相对存活率分别为38.28%和12.76%。高通量测序表明，Lc和Lp降低了斑点叉尾鮰肠道微生物群的α多样性。在益生菌处理期间， 在肠道中占主导地位，但在停止施用益生菌2周后几乎消失。与CG相比，Lc和Lp导致 的丰度增加， 的数量减少。功能分析显示，Lc处理上调了京都基因与基因组百科全书（KEGG）通路的相对丰度，包括脂质代谢、其他氨基酸代谢、萜类和聚酮类代谢、异生物质生物降解和代谢以及核苷酸代谢。综合来看，我们的数据表明，作为饲料添加剂的Lc，添加量为3.0×10 cfu/g时，可促进斑点叉尾鮰的生长性能、抗病能力，并显著改变其肠道微生物群的组成。
