Tavares Lívia A, Rezende Allan A, Santos Jymmys L, Estevam Charles S, Silva Ana M O, Schneider Jaderson K, Cunha John L S, Droppa-Almeida Daniela, Correia-Neto Ivan J, Cardoso Juliana C, Severino Patricia, Souto Eliana B, de Albuquerque-Júnior Ricardo L C
Post-Graduating Program in Biotechnology, Brazilian Biotechnology Northeast Network, Tiradentes University, Aracaju 49010-390, Brazil.
Post-Graduating Program in Biotechnology, Brazilian Biotechnology Northeast Network, Federal University of Sergipe, São Cristóvão 49100-000, Brazil.
Pharmaceutics. 2021 May 9;13(5):679. doi: 10.3390/pharmaceutics13050679.
The essential oil of (EOCW) is a natural product with antioxidant, anti-inflammatory, and antifibrotic properties. We studied the effect of EOCW in the progression of histological changes of pulmonary fibrosis (PF) in a rodent model. The oil was obtained by hydrodistillation and characterized using gas chromatography-mass spectrometry. Intratracheal instillation of bleomycin was performed in 30 rats to induce PF, while Sham animals were subjected to instillation of saline solution. The treatment was performed using daily oral administration of distilled water, EOCW at 50, 100, and 200 mg/kg, and deflazacort (DFC). After 28 days, hemogram and bronchoalveolar lavage fluid (BALF), tissue levels of malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) were assayed. Histological grading of PF, immunohistochemical expression of α-smooth muscle actin (α-SMA), and transforming growth factor-β (TGF-β) were also analyzed. The EOCW major compounds were found to be citronellal, geraniol, and citronellol. EOCW significantly reduced inflammation in BALF, reduced MDA levels, and increased SOD activity. EOCW attenuated histological grading of PF and reduced immunohistochemical expression of α-SMA and TGF-β in a dose-dependent way, likely due to the reduction of oxidative stress, inflammation, and TGF-β-induced myofibroblast differentiation.
香茅（EOCW）精油是一种具有抗氧化、抗炎和抗纤维化特性的天然产物。我们在啮齿动物模型中研究了EOCW对肺纤维化（PF）组织学变化进展的影响。该精油通过水蒸馏法获得，并采用气相色谱 - 质谱联用仪进行表征。对30只大鼠进行气管内注射博来霉素以诱导PF，而假手术动物则注射生理盐水。通过每日口服蒸馏水、50、100和200 mg/kg的EOCW以及地夫可特（DFC）进行治疗。28天后，检测血常规、支气管肺泡灌洗液（BALF）、丙二醛（MDA）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的组织水平。还分析了PF的组织学分级、α - 平滑肌肌动蛋白（α - SMA）和转化生长因子 - β（TGF - β）的免疫组化表达。发现EOCW的主要成分是香茅醛、香叶醇和香茅醇。EOCW显著减轻了BALF中的炎症，降低了MDA水平，并增加了SOD活性。EOCW以剂量依赖的方式减轻了PF的组织学分级，并降低了α - SMA和TGF - β的免疫组化表达，这可能是由于氧化应激、炎症和TGF - β诱导的肌成纤维细胞分化的减少。
