基于 NMR 代谢组学分析的对去卵巢小鼠的抗痴呆作用。

Antidementia Effects of in Ovariectomized Mice Supported by NMR-Based Metabolomic Analysis.

机构信息

Division of Pharmacognosy and Toxicology, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.

Division of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.

出版信息

Molecules. 2021 May 9;26(9):2789. doi: 10.3390/molecules26092789.

The crude ethanol extract of the whole plant of (Mart.) Griseb was investigated for its potential as antidementia, induced by estrogen deprivation, based on in vitro antioxidant activity, β-amyloid aggregation inhibition and cholinesterase inhibitory activity, as well as in vivo Morris water maze task (MWMT), novel object recognition task (NORT), and Y-maze task. To better understand the effect of the extract, oxidative stress-induced brain membrane damage through lipid peroxidation in the whole brain was also investigated. Additionally, expressions of neuroinflammatory cytokines (IL-1β, IL-6 and TNF-α) and estrogen receptor-mediated facilitation genes such as PI3K and AKT mRNA in the hippocampus and frontal cortex were also evaluated. These effects were confirmed by the determination of its serum metabolites by NMR metabolomic analysis. Both the crude extract of and its flavone constituents were found to inhibit β-amyloid (Aβ) aggregation.

采用体外抗氧化活性、β-淀粉样蛋白聚集抑制活性和乙酰胆碱酯酶抑制活性，以及体内 Morris 水迷宫任务（MWMT）、新物体识别任务（NORT）和 Y 迷宫任务，研究了（Mart.）Griseb 全植物的粗乙醇提取物作为雌激素剥夺诱导的抗痴呆的潜力。为了更好地理解提取物的作用，还通过脂质过氧化研究了全脑氧化应激诱导的细胞膜损伤。此外，还评估了海马体和前额叶皮层中神经炎性细胞因子（IL-1β、IL-6 和 TNF-α）和雌激素受体介导的促进基因（如 PI3K 和 AKT mRNA）的表达。通过 NMR 代谢组学分析确定其血清代谢物证实了这些作用。发现和其类黄酮成分的粗提取物均能抑制β-淀粉样蛋白（Aβ）聚集。