Pshenichnikova Tatyana A, Osipova Svetlana V, Smirnova Olga G, Leonova Irina N, Permyakova Marina D, Permyakov Alexey V, Rudikovskaya Elena G, Konstantinov Dmitrii K, Verkhoturov Vasiliy V, Lohwasser Ulrike, Börner Andreas
Institute of Cytology and Genetics SB RAS, 630090 Novosibirsk, Russia.
Siberian Institute of Plant Physiology and Biochemistry SB RAS, 664033 Irkutsk, Russia.
Plants (Basel). 2021 May 20;10(5):1023. doi: 10.3390/plants10051023.
Understanding the genetic architecture of drought tolerance is of great importance for overcoming the negative impact of drought on wheat yield. Earlier, we discovered the critical role of chromosome 2A for the drought-tolerant status of wheat spring cultivar Saratovskaya 29. A set of 92 single-chromosome recombinant double haploid (SCRDH) lines were obtained in the genetic background of Saratovskaya 29. The lines carry fragments of chromosome 2A from the drought-sensitive cultivar Yanetzkis Probat. The SCRDH lines were used to identify regions on chromosome 2A associated with the manifestation of physiological and agronomical traits under distinct water supply, and to identify candidate genes that may be associated with adaptive gene networks in wheat. Genotyping was done with Illumina Infinium 15k wheat array using 590 SNP markers with 146 markers being polymorphic. In four identified regions of chromosome 2A, 53 out of 58 QTLs associated with physiological and agronomic traits under contrasting water supply were mapped. Thirty-nine candidate genes were identified, of which 18 were transcription factors. The region 73.8-78.1 cM included the largest number of QTLs and candidate genes. The variation in SNPs associated with agronomical and physiological traits revealed among the SCRDH lines may provide useful information for drought related marker-assisted breeding.
了解耐旱性的遗传结构对于克服干旱对小麦产量的负面影响至关重要。此前，我们发现了2A染色体对小麦春性品种萨拉托夫斯卡亚29耐旱状态的关键作用。在萨拉托夫斯卡亚29的遗传背景下获得了一组92个单染色体重组双单倍体（SCRDH）株系。这些株系携带来自干旱敏感品种亚涅茨基斯·普罗巴特的2A染色体片段。利用SCRDH株系来鉴定2A染色体上与不同供水条件下生理和农艺性状表现相关的区域，并鉴定可能与小麦适应性基因网络相关的候选基因。使用Illumina Infinium 15k小麦芯片，利用590个SNP标记进行基因分型，其中146个标记具有多态性。在2A染色体的四个已鉴定区域中，定位了58个与不同供水条件下生理和农艺性状相关的QTL中的53个。鉴定出39个候选基因，其中18个是转录因子。73.8-78.1 cM区域包含的QTL和候选基因数量最多。SCRDH株系中与农艺和生理性状相关的SNP变异可能为干旱相关的标记辅助育种提供有用信息。
