Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
Forensic Innovation Center, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.
Molecules. 2021 May 16;26(10):2958. doi: 10.3390/molecules26102958.
A simple and highly sensitive electrochemical sensor was developed for adsorptive cathodic stripping voltammetry of alprazolam. Based on an electrochemically pretreated glassy carbon electrode, the sensor demonstrated good adsorption and electrochemical reduction of alprazolam. The morphology of the glassy carbon electrode and the electrochemically pretreated glassy carbon electrode were characterized by scanning electron microscopy/energy dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy, cyclic voltammetry, and electrochemical impedance spectroscopy. The electrochemical behaviors of alprazolam were determined by cyclic voltammetry, and the analytical measurements were studied by adsorptive cathodic stripping voltammetry. Optimized operational conditions included the concentration and deposition time of sulfuric acid in the electrochemical pretreatment, preconcentration potential, and preconcentration time. Under optimal conditions, the developed alprazolam sensor displayed a quantification limit of 0.1 mg L, a detection limit of 0.03 mg L, a sensitivity of 67 µA mg L cm and two linear ranges: 0.1 to 4 and 4 to 20 mg L. Sensor selectivity was excellent, and repeatability (%RSD < 4.24%) and recovery (82.0 ± 0.2 to 109.0 ± 0.3%) were good. The results of determining alprazolam in beverages with the developed system were in good agreement with results from the gas chromatography-mass spectrometric method.
一种简单且高灵敏的电化学传感器被开发用于阿普唑仑的吸附阴极溶出伏安法。基于电化学预处理的玻碳电极，该传感器表现出对阿普唑仑良好的吸附和电化学还原性能。玻碳电极和电化学预处理玻碳电极的形貌通过扫描电子显微镜/能量色散 X 射线光谱、傅里叶变换红外光谱、循环伏安法和电化学阻抗谱进行了表征。通过循环伏安法确定了阿普唑仑的电化学行为，并通过吸附阴极溶出伏安法研究了分析测量。优化的操作条件包括电化学预处理中硫酸的浓度和沉积时间、预浓缩电位和预浓缩时间。在最佳条件下，所开发的阿普唑仑传感器显示出 0.1 mg L 的定量限、0.03 mg L 的检测限、67 µA mg L cm 的灵敏度和两个线性范围：0.1 至 4 和 4 至 20 mg L。传感器选择性极好，重复性（%RSD < 4.24%）和回收率（82.0 ± 0.2 至 109.0 ± 0.3%）良好。用所开发的系统测定饮料中的阿普唑仑的结果与气相色谱-质谱法的结果吻合良好。
