Department of Pesticide Science, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
The Key Laboratory of Plant Immunity, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
Toxins (Basel). 2021 May 12;13(5):348. doi: 10.3390/toxins13050348.
causal agent of Fusarium head blight (FHB), causes a huge economic loss. No information is available on the activity of quinofumelin, a novel quinoline fungicide, against or other phytopathogens. In this study, we used mycelial growth and spore germination inhibition methods to determine the inhibitory effect of quinofumelin against in vitro. The results indicated that quinofumelin excellently inhibited mycelial growth and spore germination of , with the average EC values of 0.019 ± 0.007 μg/mL and 0.087 ± 0.024 μg/mL, respectively. In addition, we found that quinofumelin could significantly decrease deoxynivalenol (DON) production and inhibit the expression of DON-related gene in . Furthermore, we found that quinofumelin could disrupt the formation of Fusarium toxisome, a structure for producing DON. Western blot analysis demonstrated that the translation level of TRI1, a marker gene for Fusarium toxisome, was suppressed by quinofumelin. The protective and curative assays indicated that quinofumelin had an excellent control efficiency against on wheat coleoptiles. Taken together, quinofumelin exhibits not only an excellent antifungal activity on mycelial growth and spore germination, but also could inhibit DON biosynthesis in . The findings provide a novel candidate for controlling FHB caused by .
镰刀菌顶腐病菌（FHB）的病原菌，会导致巨大的经济损失。目前尚无关于新型喹啉类杀菌剂喹呋菌灵对该病原菌或其他植物病原菌活性的信息。在本研究中，我们使用菌丝生长和孢子萌发抑制方法来确定喹呋菌灵在体外对的抑制作用。结果表明，喹呋菌灵能极好地抑制的菌丝生长和孢子萌发，平均 EC 值分别为 0.019 ± 0.007 μg/mL 和 0.087 ± 0.024 μg/mL。此外，我们发现喹呋菌灵可以显著降低脱氧雪腐镰刀菌烯醇（DON）的产生，并抑制 DON 相关基因在中的表达。此外，我们发现喹呋菌灵可以破坏产生 DON 的产毒体的形成。Western blot 分析表明，产毒体的标记基因 TRI1 的翻译水平受到喹呋菌灵的抑制。保护和治疗试验表明，喹呋菌灵对小麦幼茎上的具有极好的防治效果。总之，喹呋菌灵不仅对菌丝生长和孢子萌发具有极好的抗真菌活性，而且可以抑制中的 DON 生物合成。这些发现为防治由引起的 FHB 提供了一种新的候选药物。
