Li Liang, Guo Nannan, Feng Yu, Duan Mengmeng, Li Chunhui
School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin, China.
Front Plant Sci. 2022 Apr 14;13:836940. doi: 10.3389/fpls.2022.836940. eCollection 2022.
Wheat is among the top 10 and most widely grown crops in the world. However, wheat is often infected with many soil-borne diseases, including sharp eyespot, mainly caused by the necrotrophic fungus , and Fusarium head blight (FHB), caused by , resulting in reduced production. is a root endophytic fungus with a wide range of host plants, which increases their growth and tolerance to biotic and abiotic stresses. In this study, the capability of to protect wheat seedlings against and was investigated at the physiological, biochemical, and molecular levels. Our results showed that significantly reduced the disease progress on wheat caused by and , but not showed any antagonistic effect on and . Additionally, can induce systemic resistance by elevating HO content, antioxidase activity, relative water content (RWC), and membrane stability index (MSI) compared to the plants only inoculated with or and control. RNA-seq suggested that transcriptome changes caused by were more severe than those caused by The number of differentially expressed genes (DEGs) in the transcriptome can be reduced by the addition of for reduced by 18% and for reduced 58%. The DEGs related to disease resistance, such as WRKY and MAPK, were upregulated by colonization. The data further revealed that the transcriptional resistance to and mediated by is quite different.
小麦是世界上排名前十且种植最广泛的作物之一。然而，小麦经常受到许多土传病害的感染，包括主要由坏死营养型真菌引起的纹枯病，以及由[具体病原菌名称缺失]引起的赤霉病，导致产量下降。[内生真菌名称缺失]是一种具有广泛寄主植物的根内生真菌，它能促进寄主植物生长并提高其对生物和非生物胁迫的耐受性。在本研究中，从生理、生化和分子水平研究了[内生真菌名称缺失]保护小麦幼苗抵御[病原菌名称缺失]和[病原菌名称缺失]的能力。我们的结果表明，[内生真菌名称缺失]显著降低了由[病原菌名称缺失]和[病原菌名称缺失]引起的小麦病害进展，但对[病原菌名称缺失]和[病原菌名称缺失]未表现出任何拮抗作用。此外，与仅接种[病原菌名称缺失]或[病原菌名称缺失]的植株以及对照相比，[内生真菌名称缺失]可通过提高过氧化氢（HO）含量、抗氧化酶活性、相对含水量（RWC）和膜稳定性指数（MSI）来诱导系统抗性。RNA测序表明，由[内生真菌名称缺失]引起的转录组变化比由[病原菌名称缺失]引起的更为严重。转录组中差异表达基因（DEG）的数量可通过添加[内生真菌名称缺失]而减少，对于[病原菌名称缺失]减少了18%，对于[病原菌名称缺失]减少了58%。与抗病性相关的DEG，如WRKY和MAPK，通过[内生真菌名称缺失]定殖而上调。数据进一步揭示，由[内生真菌名称缺失]介导的对[病原菌名称缺失]和[病原菌名称缺失]的转录抗性差异很大。
