Department of Medicine, University of Virginia School of Medicine, Charlottesville, VA 22903, USA.
College of William and Mary, Williamsburg, VA 23185, USA.
Nutrients. 2021 May 22;13(6):1768. doi: 10.3390/nu13061768.
We aimed to identify plasma and urine metabolites altered by the Dietary Approaches to Stop Hypertension (DASH) diet in a post-hoc analysis of a pilot feeding trial. Twenty adult participants with un-medicated hypertension consumed a Control diet for one week followed by 2 weeks of random assignment to either Control or DASH diet. Non-missing fasting plasma (n = 56) and 24-h urine (n = 40) were used to profile metabolites using untargeted gas chromatography/mass spectrometry. Linear models were used to compare metabolite levels between the groups. In urine, 19 identifiable untargeted metabolites differed between groups at < 0.05. These included a variety of phenolic acids and their microbial metabolites that were higher during the DASH diet, with many at false discovery rate (FDR) adjusted < 0.2. In plasma, eight identifiable untargeted metabolites were different at < 0.05, but only gamma-tocopherol was significantly lower on DASH at FDR adjusted < 0.2. The results provide insights into the mechanisms of benefit of the DASH diet.
我们旨在通过对一项初步喂养试验的事后分析，确定饮食方法阻止高血压（DASH）饮食对血浆和尿液代谢物的影响。20 名未经药物治疗的高血压成年参与者连续一周食用对照饮食，然后随机分配 2 周食用对照饮食或 DASH 饮食。使用非缺失的空腹血浆（n = 56）和 24 小时尿液（n = 40），使用非靶向气相色谱/质谱法对代谢物进行分析。线性模型用于比较组间代谢物水平。在尿液中，有 19 种可识别的非靶向代谢物在组间存在差异，差异具有统计学意义（p 值<0.05）。这些代谢物包括多种酚酸及其微生物代谢物，在 DASH 饮食期间水平更高，其中许多在 FDR 调整后<0.2。在血浆中，有 8 种可识别的非靶向代谢物在组间存在差异，差异具有统计学意义（p 值<0.05），但只有γ-生育酚在 FDR 调整后<0.2 时在 DASH 饮食中显著降低。这些结果为 DASH 饮食的益处机制提供了新的见解。
