Herrmann Jaqueline, Gummi Manasa Reddy, Xia Mengdi, van der Giet Markus, Tölle Markus, Schuchardt Mirjam
Department of Nephrology and Medical Intensive Care, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Hindenburgdamm 30, 12203 Berlin, Germany.
Department of Chemistry, Biochemistry and Pharmacy, Freie Universität Berlin, Königin-Luise-Straße 2+4, 14195 Berlin, Germany.
Biology (Basel). 2021 May 22;10(6):459. doi: 10.3390/biology10060459.
Vascular calcification is a multifaceted disease and a significant contributor to cardiovascular morbidity and mortality. The calcification deposits in the vessel wall can vary in size and localization. Various pathophysiological pathways may be involved in disease progression. With respect to the calcification diversity, a great number of research models and detection methods have been established in basic research, relying mostly on rodent models. The aim of this review is to provide an overview of the currently available rodent models and quantification methods for vascular calcification, emphasizing animal burden and assessing prospects to use available methods in a way to address the 3R principles of Russel and Burch: "Replace, Reduce and Refine".
血管钙化是一种多方面的疾病，是心血管发病和死亡的重要因素。血管壁中的钙化沉积物在大小和位置上可能有所不同。疾病进展可能涉及多种病理生理途径。关于钙化的多样性，基础研究中已经建立了大量的研究模型和检测方法，主要依赖于啮齿动物模型。本综述的目的是概述目前可用的用于血管钙化的啮齿动物模型和量化方法，强调动物负担，并评估以符合拉塞尔和伯奇的3R原则（“替代、减少和优化”）的方式使用现有方法的前景。
