Chen Yu-Hsuan, Wang Wei-Hsun, Lin Sheng-Hui, Yang-Wang Yuan-Ting, Tseng Sung-Pin, Chien Chi-Sheng, Shih Chi-Jen
School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
Department of Orthopedic Surgery, Changhua Christian Hospital, Changhua 500, Taiwan.
Polymers (Basel). 2021 May 10;13(9):1529. doi: 10.3390/polym13091529.
() is a common and challenging pathogen of nosocomial infections, due to its ability to survive on inanimate objects, desiccation tolerance, and resistance to disinfectants. In this study, we investigated an antibacterial strategy to combat via the combination of antibiotics and silver protein. This strategy used a functional platform consisting of silver nanoparticles (AgNPs) resurrected from silver-based calcium thiophosphate (SSCP) through casein and arginine. Then, the silver protein was combined with tigecycline, the first drug in glycylcycline antibiotic, to synergistically inhibit the viability of . The synergistic antibacterial activity was confirmed by the 96-well checkerboard method to determine their minimum inhibitory concentrations (MIC) and calculated for the combination index (CI). The MIC of the combination of silver protein and tigecycline (0.31 mg/mL, 0.16 µg/mL) was significantly lower than that of the individual MIC, and the CI was 0.59, which indicates a synergistic effect. Consequently, we integrated the detailed synergistic antibacterial properties when silver protein was combined with tigecycline. The result could make for a promising approach for the treatment of .
（）是医院感染中一种常见且具有挑战性的病原体，因其能够在无生命物体上存活、耐受干燥以及对消毒剂具有抗性。在本研究中，我们研究了一种通过抗生素与银蛋白联合来对抗（）的抗菌策略。该策略使用了一个功能平台，该平台由通过酪蛋白和精氨酸从硫代磷酸钙银（SSCP）中复活的银纳米颗粒（AgNP）组成。然后，将银蛋白与甘氨酰环素类抗生素中的第一种药物替加环素联合，以协同抑制（）的活力。通过96孔棋盘法确定它们的最低抑菌浓度（MIC）并计算联合指数（CI），从而证实了协同抗菌活性。银蛋白与替加环素组合（0.31mg/mL，0.16μg/mL）的MIC显著低于各自的MIC，且CI为0.59，这表明具有协同作用。因此，我们整合了银蛋白与替加环素联合时详细的协同抗菌特性。该结果可能为（）的治疗提供一种有前景的方法。
