Division of Gastroenterology (General Medicine), Department of Internal Medicine, Yuan's General Hospital, No.162, Cheng Kung 1st Rd., Lingya District, Kaohsiung 80249, Taiwan.
Division of Hematology and Oncology, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80756, Taiwan.
Mar Drugs. 2021 May 14;19(5):275. doi: 10.3390/md19050275.
Fish gelatin and its hydrolysates exhibit a variety of biological characteristics, which include antihypertensive and antioxidant properties. In this study, fish gelatins were extracted from extrusion-pretreated tilapia scales, and then subjected to analyses to determine the physicochemical properties and antioxidant activity of the extracted gelatins. Our findings indicate that TSG2 (preconditioned with 1.26% citric acid) possessed the greatest extraction yield, as well as higher antioxidant activities compared with the other extracted gelatins. Hence, TSG2 was subjected to further hydrolyzation using different proteases and ultrafiltration conditions, which yielded four gelatin hydrolysates: TSGH1, TSGH2, TSGH3, and TSGH4. The results showed that TSGH4 (Pepsin + Pancreatin and ultrafiltration < 3000 Da) had a higher yield and greater antioxidant activity in comparison with the other gelatin hydrolysates. As such, TSGH4 was subjected to further fractionation using a Superdex peptide column and two-stage reverse-phase column HPLC chromatography, yielding a subfraction TSGH4-6-2-b, which possessed the highest 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity compared with the other fractions. Further LC-ESI/MS/MS analysis of TSGH4-6-2-b suggested two novel peptides (GYDEY and EPGKSGEQGAPGEAGAP), which could have potential as naturally-occurring peptides with antioxidant properties. These promising results suggest that these antioxidant peptides could have applications in food products, nutraceuticals, and cosmetics.
鱼明胶及其水解产物具有多种生物学特性，包括降压和抗氧化特性。在这项研究中，从挤压预处理罗非鱼鳞中提取鱼明胶，然后对其进行分析，以确定提取明胶的理化性质和抗氧化活性。我们的研究结果表明，TSG2（用 1.26%柠檬酸预处理）的提取率最高，并且与其他提取的明胶相比，具有更高的抗氧化活性。因此，TSG2 进一步用不同的蛋白酶和超滤条件进行水解，得到四种明胶水解产物：TSGH1、TSGH2、TSGH3 和 TSGH4。结果表明，与其他明胶水解产物相比，TSGH4（胃蛋白酶+胰蛋白酶和超滤<3000 Da）具有更高的产率和更强的抗氧化活性。因此，TSGH4 进一步用 Superdex 肽柱和两步反相柱 HPLC 色谱进行分段，得到一个亚组分 TSGH4-6-2-b，与其他亚组分相比，具有最高的 2,2-二苯基-1-苦基肼（DPPH）清除活性。对 TSGH4-6-2-b 的进一步 LC-ESI/MS/MS 分析表明，存在两种新型肽（GYDEY 和 EPGKSGEQGAPGEAGAP），它们可能具有作为天然存在的抗氧化肽的应用潜力。这些有前途的结果表明，这些抗氧化肽可应用于食品、营养保健品和化妆品。
