State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, 730046, China.
Qinghai Provincial Center for Animal Disease Control and Prevention, Xining, 810003, China.
Infect Dis Poverty. 2019 Feb 7;8(1):12. doi: 10.1186/s40249-019-0522-z.
Anaplasma species are tick-transmitted obligate intracellular bacteria that infect many wild and domestic animals and humans. The prevalence of Anaplasma spp. in ixodid ticks of Qinghai Province is poorly understood. In this study, a total of 1104 questing adult ticks were investigated for the infection of Anaplasma species. As a result, we demonstrated the total infection rates of 3.1, 11.1, 5.6, and 4.5% for A. phagocytophilum, A. bovis, A. ovis and A. capra, respectively. All of the tick samples were negative for A. marginale. The positive rates of A. phagocytophilum, A. ovis and A. capra in different tick species were significantly different. The positive rates of A. capra and A. bovis in the male ticks were significantly higher than that in the female ticks. Sequence analysis of A. ovis showed 99.5-100% identity to the previous reported isolates. The sequences of A. phagocytophilum had 100% identity to strains Ap-SHX21, JC3-3 and ZAM dog-181 from sheep, Mongolian gazelles, and dogs. Two genotypes of A. capra were found based on 16S rRNA, citrate synthase (gltA) gene and heat shock protein (groEL) gene analysis. In conclusion, A. bovis, A. ovis, A. phagocytophilum, and A. capra were present in the ticks in Qinghai Province. Anaplasma infection is associated with tick species, gender and distribution. These data will be helpful for understanding prevalence status of Anaplasma infections in ticks in Qinghai-Tibet Plateau.
无形体属细菌是经蜱传播的专性细胞内寄生菌，可感染许多野生动物和家养动物以及人类。青海省硬蜱中无形体属的流行情况尚不清楚。在本研究中，共调查了 1104 只采集的成年蜱，以检测无形体属的感染情况。结果显示，A. phagocytophilum、A. bovis、A. ovis 和 A. capra 的总感染率分别为 3.1%、11.1%、5.6%和 4.5%。所有蜱样本均为 A. marginale 阴性。不同蜱种中 A. phagocytophilum、A. ovis 和 A. capra 的阳性率有显著差异。雄蜱中 A. capra 和 A. bovis 的阳性率明显高于雌蜱。A. ovis 的序列分析与之前报道的分离株具有 99.5-100%的同一性。A. phagocytophilum 的序列与来自绵羊、蒙古瞪羚和狗的 Ap-SHX21、JC3-3 和 ZAM dog-181 株具有 100%的同一性。根据 16S rRNA、柠檬酸合酶（gltA）基因和热休克蛋白（groEL）基因分析，发现了两种 A. capra 基因型。总之，青海省蜱中存在 A. bovis、A. ovis、A. phagocytophilum 和 A. capra。无形体感染与蜱种、性别和分布有关。这些数据将有助于了解青藏高原蜱中无形体感染的流行情况。
