Xu Junping, Ahn Chang Ho, Shin Ju Young, Park Pil Man, An Hye Ryun, Kim Yae-Jin, Lee Su Young
Rural Development Administration, Floriculture Research Division, National Institute of Horticultural and Herbal Science, Wanju 55365, Korea.
Garden Plant Material Development Team, Korea Institute of Arboretum Management, Sejong 30106, Korea.
Plants (Basel). 2021 May 19;10(5):1011. doi: 10.3390/plants10051011.
Toluene is an industrial raw material and solvent that can be found abundantly in our daily life products. The amount of toluene vapor is one of the most important measurements for evaluating air quality. The evaluation of toluene scavenging ability of different plants has been reported, but the mechanism of plant response to toluene is only partially understood. In this study, we performed RNA sequencing (RNA-seq) analysis to detect differential gene expression in toluene-treated and untreated leaves of . A total of 88,444 unigenes were identified by RNA-seq analysis, of which 49,623 were successfully annotated and 4101 were differentially expressed. Gene ontology analysis revealed several subcategories of genes related to toluene response, including cell part, cellular process, organelle, and metabolic processes. We mapped the main metabolic pathways of genes related to toluene response and found that the differentially expressed genes were mainly involved in glycolysis/gluconeogenesis, starch and sucrose metabolism, glycerophospholipid metabolism, carotenoid biosynthesis, phenylpropanoid biosynthesis, and flavonoid biosynthesis. In addition, 53 transcription factors belonging to 13 transcription factor families were identified. We verified 10 differentially expressed genes related to metabolic pathways using quantitative real-time PCR and found that the results of RNA-seq were positively correlated with them, indicating that the transcriptome data were reliable. This study provides insights into the metabolic pathways involved in toluene response in plants.
甲苯是一种工业原料和溶剂，在我们的日常生活用品中大量存在。甲苯蒸汽量是评估空气质量的最重要指标之一。已有报道对不同植物清除甲苯的能力进行了评估，但植物对甲苯的响应机制仅得到部分了解。在本研究中，我们进行了RNA测序（RNA-seq）分析，以检测经甲苯处理和未经处理的叶片中的差异基因表达。通过RNA-seq分析共鉴定出88,444个单基因，其中49,623个成功注释，4101个差异表达。基因本体分析揭示了与甲苯响应相关的几个基因亚类，包括细胞部分、细胞过程、细胞器和代谢过程。我们绘制了与甲苯响应相关基因的主要代谢途径，发现差异表达基因主要参与糖酵解/糖异生、淀粉和蔗糖代谢、甘油磷脂代谢、类胡萝卜素生物合成、苯丙烷生物合成和类黄酮生物合成。此外，鉴定出属于13个转录因子家族的53个转录因子。我们使用定量实时PCR验证了10个与代谢途径相关的差异表达基因，发现RNA-seq结果与它们呈正相关，表明转录组数据可靠。本研究为植物中甲苯响应所涉及的代谢途径提供了见解。
