Vuko Elma, Dunkić Valerija, Ruščić Mirko, Nazlić Marija, Mandić Nela, Soldo Barbara, Šprung Matilda, Fredotović Željana
Department of Biology, Faculty of Science, University of Split, R. Boškovića 33, 21000 Split, Croatia.
Department of Chemistry, Faculty of Science, University of Split, R. Boškovića 33, 21000 Split, Croatia.
Plants (Basel). 2021 May 19;10(5):1014. doi: 10.3390/plants10051014.
The chemical profile, antiproliferative, antioxidant and antiphytoviral activities of the species ssp. (Schrank) H. Lindb. (Clusiaceae) were investigated. Free volatiles were isolated and the chemical composition was determined in the lipophilic fraction (essential oil) and for the first time in the water fraction (hydrosol). The aim is to provide phytochemical data for ssp. useful for distinguishing ssp. from ssp. , as there are taxonomic disagreements between them and the composition of the secretory products may be helpful in this respect. In the essential oil, the most abundant compounds identified were α-pinene and -nonane, while in the hydrosol, myrtenol, carvacrol and α-pinene were the most abundant. Overall, the class of monoterpenes and oxygenated monoterpenes dominated in the EO and hydrosol samples. The essential oil showed high antioxidant activity, in contrast to the antiproliferative activity, where the hydrosol showed exceptional activity against three cancer cell lines: Hela (cervical cancer cell line), HCT116 (human colon cancer cell line) and U2OS (human osteosarcoma cell line). Both the essential oil and hydrosol showed antiphytoviral activity against tobacco mosaic virus infection on the local host plants. This is the first report dealing with biological activities of hydrosol of ssp. , and the obtained results suggest that this traditional medicinal plant is a valuable source of volatiles with promising antiproliferative, antioxidant and antiphytoviral activities.
对（Schrank）H. Lindb.（藤黄科）物种的化学特征、抗增殖、抗氧化和抗植物病毒活性进行了研究。分离出游离挥发物，并确定了亲脂性部分（精油）以及首次确定了水性部分（水溶胶）的化学成分。目的是为该物种提供植物化学数据，这有助于将其与其他物种区分开来，因为它们之间存在分类学上的分歧，而分泌产物的组成在这方面可能会有所帮助。在精油中，鉴定出的最丰富化合物是α-蒎烯和壬烷，而在水溶胶中，桃金娘烯醇、香芹酚和α-蒎烯最为丰富。总体而言，单萜类和氧化单萜类在精油和水溶胶样品中占主导地位。精油显示出高抗氧化活性，与抗增殖活性形成对比，在抗增殖活性方面，水溶胶对三种癌细胞系表现出异常活性：Hela（宫颈癌细胞系）、HCT116（人结肠癌细胞系）和U2OS（人骨肉瘤细胞系）。精油和水溶胶对局部寄主植物上的烟草花叶病毒感染均显示出抗植物病毒活性。这是关于该物种水溶胶生物活性的首次报道，所得结果表明这种传统药用植物是具有有前景的抗增殖、抗氧化和抗植物病毒活性的挥发物的宝贵来源。
