Fageeh Hytham N, Bhandi Shilpa, Mashyakhy Mohammed, Kahtani Ahmed Al, Badran Zahi, Mehta Deepak, Fageeh Hammam Ibrahim, Balaji Thodur Madapusi, Baeshen Hosam Ali, Varadarajan Saranya, Raj A Thirumal, Patil Vikrant R, Vyas Nishant, Zanza Alessio, Testarelli Luca, Patil Shankargouda
Department of Preventive Dental Sciences, College of Dentistry, Jazan University, Jazan 45142, Saudi Arabia.
Department of Restorative Dental Sciences, College of Dentistry, Jazan University, Jazan 45142, Saudi Arabia.
J Pers Med. 2021 May 18;11(5):430. doi: 10.3390/jpm11050430.
Autogenous gingival grafts used for root coverage or gingival augmentation procedures often result in donor site morbidity. Living cellular constructs as an exogenous alternative have been proven to be associated with lower morbidity. With the available background information, the present study aims to assess if quercetin-induced living cell constructs, derived from dental pulp stem cells, have the potential to be applied as a tool for soft tissue augmentation. The characterized dental pulp stem cells (positive for CD73, CD90, and negative for CD34, HLA-DR) were expanded in Dulbecco's Modified Eagle's medium (DMEM) supplemented with 10 mM quercetin. The handling properties of the quercetin-induced dental pulp stem cell constructs were assessed by visual, and tactile sensation. A microscopic characterization using hematoxylin and eosin staining, and qRT-PCR-based analysis for stemness-associated genes (OCT4, NANOG, SOX2, and cMyc) was also performed. Dental pulp stem cells without quercetin administration were used as the control. Dental pulp stem cell constructs induced by quercetin easily detached from the surface of the plate, whereas there was no formation in the control cells. It was also simple to transfer the induced cellular construct on the flattened surface. Microscopic characterization of the constructs showed cells embedded in a tissue matrix. Quercetin also increased the expression of stemness-related genes. The use of quercetin-induced DPSC living constructs for soft tissue augmentation could provide an alternative to autogenous soft tissue grafts to lower patient morbidity and improve esthetic outcomes.
用于牙根覆盖或牙龈增厚手术的自体牙龈移植术常常会导致供体部位出现病变。作为一种外源性替代方法的活细胞构建体已被证明与较低的病变发生率相关。基于现有的背景信息，本研究旨在评估源自牙髓干细胞的槲皮素诱导的活细胞构建体是否有潜力作为软组织增厚的一种工具来应用。对已鉴定的牙髓干细胞（CD73、CD90呈阳性，CD34、HLA-DR呈阴性）在补充有10 mM槲皮素的杜氏改良 Eagle 培养基（DMEM）中进行扩增。通过视觉和触觉感受来评估槲皮素诱导的牙髓干细胞构建体的操作特性。还使用苏木精和伊红染色进行了微观表征，并基于qRT-PCR对干性相关基因（OCT4、NANOG、SOX2和cMyc）进行了分析。未给予槲皮素的牙髓干细胞用作对照。槲皮素诱导的牙髓干细胞构建体很容易从平板表面分离，而对照细胞中未形成这种构建体。将诱导的细胞构建体转移到平坦表面上也很简单。构建体的微观表征显示细胞嵌入组织基质中。槲皮素还增加了干性相关基因的表达。使用槲皮素诱导的牙髓干细胞活构建体进行软组织增厚可为自体软组织移植提供一种替代方法，以降低患者的病变发生率并改善美学效果。
