Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 40402, Taiwan.
School of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung 40402, Taiwan.
Biomolecules. 2021 May 30;11(6):812. doi: 10.3390/biom11060812.
Chinese herbal remedies have long been used for enhancing immunity and treating asthma. However, the evidence-based efficacy remains to be supported. This study aimed to explore the potential bio-signatures in allergic asthma and the effect of (YGW), a traditional Chinese herbal prescription, on dust mite-induced mouse allergic asthma. Extract of (), a dust mite, was intratracheally administered to induce allergic asthma in mice. Serum metabolomic and 16S rRNA-based microbiome profiling were used to analyze untargeted metabolites with levels significantly changed and gut microbiota composition, respectively. Results indicated that 10 metabolites (acetylcarnitine, carnitine, hypoxanthine, tryptophan, phenylalanine, norleucine, isoleucine, betaine, methionine, and valine), mainly associated with branched-chain amino acid (BCAA) metabolism, aromatic amino acid (AAA) biosynthesis, and phenylalanine metabolism were markedly elevated after treatment. YGW administration reversed the levels for 7 of the 10 identified metabolites, chiefly affecting BCAA metabolism. On 16S DNA sequencing, disordered -induced gut microbiota was significantly alleviated by YGW. Multiple correlation analysis showed a good correlation between gut microbiota composition and levels of selected metabolites. Our study showed YGW administration effectively alleviated BCAA metabolic disorder and improved gut dysbiosis. This study provides support for YGW administration with benefits for allergic asthma.
中草药长期以来一直被用于增强免疫力和治疗哮喘。然而，其基于证据的疗效仍有待支持。本研究旨在探讨过敏性哮喘的潜在生物标志物，以及中药方剂（YGW）对尘螨诱导的小鼠过敏性哮喘的影响。用尘螨（）提取物经气管内给药诱导小鼠发生过敏性哮喘。采用血清代谢组学和 16S rRNA 微生物组分析分别分析水平显著改变的非靶向代谢物和肠道微生物组成。结果表明，10 种代谢物（乙酰肉碱、肉碱、次黄嘌呤、色氨酸、苯丙氨酸、正亮氨酸、异亮氨酸、甜菜碱、蛋氨酸和缬氨酸）主要与支链氨基酸（BCAA）代谢、芳香族氨基酸（AAA）生物合成和苯丙氨酸代谢有关，在 治疗后显著升高。YGW 给药后，10 种鉴定代谢物中有 7 种的水平得到逆转，主要影响 BCAA 代谢。在 16S DNA 测序中，YGW 显著缓解了 - 诱导的肠道菌群失调。多元相关分析显示，肠道微生物组成与选定代谢物的水平之间存在良好的相关性。本研究表明，YGW 给药可有效缓解 BCAA 代谢紊乱，改善肠道菌群失调。这项研究为 YGW 给药治疗过敏性哮喘提供了支持。
