Department of Food Science, National Ilan University, Number 1, Section 1, Shen-Lung Road, Yilan City 260007, Taiwan.
Molecules. 2021 May 30;26(11):3297. doi: 10.3390/molecules26113297.
Extracts from can cause neural cells to produce nerve growth factor (NGF) and protect against neuron death. The objective of this study was to evaluate the effects of ethanol and hot water extracts from solid-state fermented wheat product on the brain cells of zebrafish embryos in both pre-dosing protection mode and post-dosing repair mode. The results showed that 1% ethanol could effectively promote zebrafish embryo brain cell death. Both 200 ppm of ethanol and water extracts from solid-state fermented wheat product protected brain cells and significantly reduced the death of brain cells caused by 1% ethanol treatment in zebrafish. Moreover, the zebrafish embryos were immersed in 1% ethanol for 4 h to cause brain cell damage and were then transferred and soaked in the 200 ppm of ethanol and water extracts from solid-state fermented wheat product to restore the brain cells damaged by the 1% ethanol. However, the 200 ppm extracts from the unfermented wheat medium had no protective and repairing effects. Moreover, 200 ppm of ethanol and water extracts from fruiting body had less significant protective and restorative effects on the brain cells of zebrafish embryos. Both the ethanol and hot water extracts from solid-state fermented wheat product could protect and repair the brain cells of zebrafish embryos damaged by 1% ethanol. Therefore, it has great potential as a raw material for neuroprotective health product.
从 中提取的物质可以促使神经细胞产生神经生长因子（NGF），并防止神经元死亡。本研究的目的是评估固态发酵小麦产品的乙醇和热水提取物在预给药保护模式和后给药修复模式下对斑马鱼胚胎脑细胞的影响。结果表明，1%乙醇能有效促进斑马鱼胚胎脑细胞死亡。200ppm 的乙醇和固态发酵小麦产品的水提取物都能保护脑细胞，并显著减少 1%乙醇处理对斑马鱼脑细胞死亡的影响。此外，将斑马鱼胚胎浸泡在 1%乙醇中 4 小时会导致脑细胞损伤，然后转移并浸泡在 200ppm 的乙醇和固态发酵小麦产品的水提取物中，以恢复 1%乙醇损伤的脑细胞。然而，未发酵小麦培养基的 200ppm 提取物对脑细胞没有保护和修复作用。此外，200ppm 的 子实体的乙醇和水提取物对斑马鱼胚胎脑细胞的保护和修复作用不明显。固态发酵小麦产品的乙醇和热水提取物都可以保护和修复 1%乙醇损伤的斑马鱼胚胎脑细胞。因此，它作为一种神经保护保健品的原料具有很大的潜力。
