过表达胆碱氧化酶基因烟草植株对NaCl敏感性的形态生理测试

Morpho-Physiological Testing of NaCl Sensitivity of Tobacco Plants Overexpressing Choline Oxidase Gene.

作者信息

Raldugina Galina N, Evsukov Sergey V, Bogoutdinova Liliya R, Gulevich Alexander A, Baranova Ekaterina N

机构信息

K. A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia.

Plant Cell Biology Laboratory, All-Russia Research Institute of Agricultural Biotechnology, Russian Academy of Sciences, Timiryazevskaya Street 42, 127550 Moscow, Russia.

出版信息

Plants (Basel). 2021 May 30;10(6):1102. doi: 10.3390/plants10061102.

In this study the transgenic lines (TLs) of tobacco ( L.), which overexpress the heterologous gene encoding the bacterial enzyme choline oxidase were evaluated. The goal of our work is to study the effect of choline oxidase gene expression on the sensitivity of plant tissues to the action of NaCl. The regenerative capacity, rhizogenesis, the amount of photosynthetic pigments and osmotically active compounds (proline and glycine betaine) were assessed by in vitro cell culture methods using biochemical and morphological parameters. Transgenic lines with confirmed expression were characterized by high regeneration capacity from callus in the presence of 200 mmol NaCl, partial retention of viability at 400 mmol NaCl. These data correlated with the implicit response of regenerants and whole plants to the harmful effects of salinity. They turned out to be less sensitive to the presence of 200 mmol NaCl in the cultivation medium, in contrast to the WT plants.

在本研究中，对过表达编码细菌胆碱氧化酶的异源基因的烟草转基因株系进行了评估。我们工作的目标是研究胆碱氧化酶基因表达对植物组织对NaCl作用敏感性的影响。使用生化和形态学参数，通过体外细胞培养方法评估再生能力、生根、光合色素和渗透活性化合物（脯氨酸和甘氨酸甜菜碱）的含量。经确认表达的转基因株系的特征是，在200 mmol NaCl存在下，愈伤组织具有高再生能力，在400 mmol NaCl时部分保持活力。这些数据与再生植株和整株植物对盐害的隐性反应相关。结果表明，与野生型植株相比，它们对培养基中200 mmol NaCl的存在不太敏感。