Department of Maxillofacial Surgery and Diagnostic Sciences, Division of Oral Medicine, College of Dentistry, Jazan University, Jazan 45142, Saudi Arabia.
Department of Maxillofacial Surgery and Diagnostic Sciences, Division of Oral Pathology, College of Dentistry, Jazan University, Jazan 45142, Saudi Arabia.
Curr Issues Mol Biol. 2021 May 23;43(1):187-196. doi: 10.3390/cimb43010016.
To evaluate the effects of allicin on mediators of pain secreted by oral cancer cells in vitro, single-cell suspensions were prepared by enzymatic method from oral squamous cell carcinoma (OSCC). Cancer stem cells were isolated by the CD133+ selection method with magnetic cell sorting. Stemness markers were checked in both cancer cells and cancer stem cells by RT-PCR. Comparative analysis of pain mediators TNF-alpha, IL-8, and endothelin at both RNA and protein levels for normal epithelial cells, cancer cells, and cancer stem cells was carried out with and without allicin treatment. CD133 and CD44 expression levels were checked in cancer cells and cancer stem cells flow cytometrically. Allicin inhibited both gene and protein expression of TNF-alpha, IL-8, and endothelin in both cancer cells and cancer stem cells. Allicin is more likely to be a promising treatment in alleviating the levels of pain and inflammation in OSCCs.
为了评估大蒜素对体外口腔癌细胞分泌的疼痛介质的影响，通过酶法从口腔鳞状细胞癌（OSCC）中制备单细胞悬液。通过磁细胞分选的 CD133+选择方法分离癌症干细胞。通过 RT-PCR 检查两种癌细胞和癌症干细胞中的干性标志物。在有和没有大蒜素处理的情况下，对正常上皮细胞、癌细胞和癌症干细胞的疼痛介质 TNF-α、IL-8 和内皮素进行 RNA 和蛋白质水平的比较分析。通过流式细胞术检查癌细胞和癌症干细胞中的 CD133 和 CD44 表达水平。大蒜素抑制了癌细胞和癌症干细胞中 TNF-α、IL-8 和内皮素的基因和蛋白表达。大蒜素更有可能成为缓解 OSCC 疼痛和炎症水平的有前途的治疗方法。
