Akahane Hugo Genki Kagawa, Gomes Ricardo Zanetti, Paludo Katia Sabrina, Linhares Filipe, Lopes Luana
Graduate student, School of Medicine, Universidade Estadual de Ponta Grossa (UEPG), Brazil. Scientific and intellectual content of the study, acquisition of data, technical procedures, manuscript preparation.
PhD, Head, Department of Medicine, UEPG, Ponta Grossa-PR, Brazil. Scientific, intellectual, conception and design of the study; interpretation of data; critical revision.
Acta Cir Bras. 2017 Sep;32(9):746-754. doi: 10.1590/s0102-865020170090000007.
To analyze the effects of allopurinol and of post-conditioning on lung injuries induced by lower-limb ischemia and reperfusion.
Thirty rats were used. They were divided in 5 groups: (1) group A: abdominal aortic dissection only, (2) group B: ischemia and reperfusion, (3) group C: administered allopurinol (100mg/Kg) a few hours before procedure, (4) group D: post-conditioned and (5) group E: administered allopurinol and post-conditioned. With the exception of group A, all groups were submitted to infrarenal aortic ischemia for 2 hours, and reperfusion for 72 hours. After euthanasia, lungs were removed for histological analysis. They were graded under two scores: pulmonary injury (neutrophil infiltration, interstitial edema, vascular congestion, and destruction of lung architecture) and lymphocytic score (neutrophil infiltration, lymphoid aggregate and secondary follicle).
On the pulmonary injury score, the degree of injury was smaller than in groups D and E, when compared to group B, p<0.05. Group C did not obtain the same result (p>0,05). On the lymphocytic score, there was no statistic difference among groups, p>0.05.
Both post-conditioning and the combination of allopurinol and post-conditioning were effective in remote lung protection induced by lower-limbs I/R. When used in isolation, allopurinol showed no protective effect.
分析别嘌醇及后处理对下肢缺血再灌注所致肺损伤的影响。
采用30只大鼠，分为5组：（1）A组：仅行腹主动脉分离术；（2）B组：缺血再灌注组；（3）C组：在手术前数小时给予别嘌醇（100mg/kg）；（4）D组：后处理组；（5）E组：给予别嘌醇并进行后处理。除A组外，其余各组均行肾下主动脉缺血2小时，再灌注72小时。安乐死后，取出肺组织进行组织学分析。根据两项评分进行分级：肺损伤（中性粒细胞浸润、间质水肿、血管充血和肺结构破坏）和淋巴细胞评分（中性粒细胞浸润、淋巴聚集和次级滤泡）。
在肺损伤评分方面，与B组相比，D组和E组的损伤程度较小，p<0.05。C组未获得相同结果（p>0.05）。在淋巴细胞评分方面，各组之间无统计学差异，p>0.05。
后处理以及别嘌醇与后处理联合应用对下肢缺血/再灌注诱导的远隔肺保护均有效。单独使用别嘌醇无保护作用。
