奥地利猪群中林达病毒中和抗体的流行率。

Prevalence of Linda Virus Neutralizing Antibodies in the Austrian Pig Population.

机构信息

Department for Pathobiology, Institute of Virology, University of Veterinary Medicine, Veterinaerplatz 1, 1210 Vienna, Austria.

Department for Farm Animals and Veterinary Public Health, University Clinic for Swine, University of Veterinary Medicine, Veterinaerplatz 1, 1210 Vienna, Austria.

出版信息

Viruses. 2021 May 27;13(6):1001. doi: 10.3390/v13061001.

DOI:10.3390/v13061001

PMID:34071946

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8229103/

Abstract

A novel pestivirus species, termed Lateral-shaking Inducing Neuro-Degenerative Agent virus (LindaV), was discovered in a piglet-producing farm in Austria in 2015 related to severe congenital tremor cases. Since the initial outbreak LindaV has not been found anywhere else. In this study, we determined the seroprevalence of LindaV infections in the domestic pig population of Austria. A fluorophore labeled infectious cDNA clone of LindaV (mCherry-LindaV) was generated and used in serum virus neutralization (SVN) assays for the detection of LindaV specific neutralizing antibodies in porcine serum samples. In total, 637 sera from sows and gilts from five federal states of Austria, collected between the years 2015 and 2020, were analyzed. We identified a single serum showing a high neutralizing antibody titer, that originated from a farm (Farm S2) in the proximity of the initially affected farm. The analysis of 57 additional sera from Farm S2 revealed a wider spread of LindaV in this pig herd. Furthermore, a second LindaV strain originating from this farm could be isolated in cell culture and was further characterized at the genetic level. Possible transmission routes and virus reservoir hosts of this emerging porcine virus need to be addressed in future studies.

摘要

一种新型的瘟病毒物种，称为 lateral-shaking inducing neurodegenerative agent virus（LindaV），于 2015 年在奥地利的一个仔猪养殖场中被发现，与严重的先天性震颤病例有关。自最初爆发以来，LindaV 尚未在其他任何地方发现。在本研究中，我们确定了奥地利国内猪群中 LindaV 感染的血清流行率。生成了 LindaV 的荧光标记传染性 cDNA 克隆（mCherry-LindaV），并将其用于血清病毒中和（SVN）测定中，以检测猪血清样品中的 LindaV 特异性中和抗体。总共分析了来自奥地利五个联邦州的 637 份来自母猪和小母猪的血清，这些血清采集于 2015 年至 2020 年之间。我们发现了一个单一的血清显示出高中和抗体滴度，该血清来自最初受影响的农场附近的一个农场（Farm S2）。对来自 Farm S2 的另外 57 个血清的分析显示，这种 LindaV 在这个猪群中传播得更广泛。此外，还可以从该农场分离出第二种 LindaV 株，并在细胞培养中进一步进行遗传水平的表征。在未来的研究中，需要解决这种新出现的猪病毒的可能传播途径和病毒储存宿主。