Institute of Virology, Department for Pathobiology, University of Veterinary Medicine, Veterinaerplatz 1, 1210 Vienna, Austria.
Department for Farm Animals and Veterinary Public Health, University Clinic for Swine, University of Veterinary Medicine, Veterinaerplatz 1, 1210 Vienna, Austria.
Viruses. 2019 Oct 23;11(11):975. doi: 10.3390/v11110975.
The novel pestivirus species known as lateral-shaking inducing neuro-degenerative agent (LINDA) virus emerged in 2015 in a piglet-producing farm in Austria. Affected piglets showed strong congenital tremor as a result of severe lesions in the central nervous system. Here, we report the results of a controlled animal infection experiment. Post-weaning piglets were infected with LINDA to determine the susceptibility of pigs, the clinical consequences of infection and the humoral immune response against LINDA. No clinically overt disease signs were observed in the piglets. Viremia was hardly detectable, but LINDA was present in the spleen and several lymphatic organs until the end of the experiment on day 28 post-infection. Oronasal virus shedding together with the infection of one sentinel animal provided additional evidence for the successful replication and spread of LINDA in the piglets. Starting on day 14 post-infection, all infected animals showed a strong humoral immune response with high titers of neutralizing antibodies against LINDA. No cross-neutralizing activity of these sera with other pestiviral species was observed. According to these data, following postnatal infection, LINDA is a rather benign virus that can be controlled by the pig's immune system. However, further studies are needed to investigate the effects of LINDA on the fetus after intrauterine infection.
新型瘟病毒种，即侧向摇晃诱导神经退行性剂（LINDA）病毒，于 2015 年在奥地利一家仔猪生产农场出现。受感染的仔猪由于中枢神经系统严重受损，表现出强烈的先天性震颤。在此，我们报告了一项受控动物感染实验的结果。感染 LINDA 的断奶仔猪用于确定猪的易感性、感染的临床后果以及针对 LINDA 的体液免疫反应。仔猪未出现明显的临床疾病迹象。病毒血症几乎检测不到，但 LINDA 存在于脾脏和几个淋巴器官中，直到感染后第 28 天实验结束。口鼻病毒脱落以及一只哨兵动物的感染为 LINDA 在仔猪中成功复制和传播提供了额外的证据。从感染后第 14 天开始，所有感染动物均表现出强烈的体液免疫反应，针对 LINDA 的中和抗体滴度很高。这些血清与其他瘟病毒种无交叉中和活性。根据这些数据，出生后感染 LINDA 是一种相当良性的病毒，可以被猪的免疫系统控制。然而，需要进一步研究来调查 LINDA 对宫内感染后胎儿的影响。
