猪脐静脉血和脐动脉血中微小RNA的表达特征

Expression Characteristics of microRNA in Pig Umbilical Venous Blood and Umbilical Arterial Blood.

作者信息

Gan Mailin, Liu Lin, Zhang Shunhua, Guo Zongyi, Tan Ya, Luo Jia, Yang Qiong, Pan Hongmei, Li Xuewei, Wang Jinyong, Shen Linyuan, Zhu Li

机构信息

College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.

Farm Animal Genetic Resource Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.

出版信息

Animals (Basel). 2021 May 27;11(6):1563. doi: 10.3390/ani11061563.

DOI:10.3390/ani11061563

PMID:34071966

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8228062/

Abstract

As the medium of material exchange between mother and fetus, umbilical cord blood is closely connected with fetal development. microRNA (miRNA) has a wide range of biological functions and has high flow characteristics. Small RNA sequencing of pig umbilical venous blood (UVB) and umbilical arterial blood (UAB) revealed that a total of 302 miRNAs were identified, and 106 and 22 miRNAs were specifically expressed in the UVB and UAB, respectively. Using the two methods of differential expression multiple and differential expression percentage, it is found that only 35% of the highly expressed miRNAs in the UVB by the two analysis modes overlap, but 56.25% of the enriched signal pathways are the same. Only 20% of the highly expressed miRNAs in the UAB overlap, but 62.07% of the signal pathways are the same. Further analysis revealed that miR-423 can be used as a characteristic miRNA of UVB and has the potential to treat muscle-related diseases. miR-122-5p can be used as a characteristic miRNA of UAB and may help to improve liver- and brain-related diseases. In summary, these results enrich understanding of miRNA in mother-fetal communication and provide a reference for the development and application of porcine cord blood products.

摘要

作为母胎之间物质交换的媒介，脐带血与胎儿发育密切相关。微小RNA（miRNA）具有广泛的生物学功能且具有高丰度特性。对猪脐静脉血（UVB）和脐动脉血（UAB）进行小RNA测序发现，共鉴定出302种miRNA，其中分别有106种和22种miRNA在UVB和UAB中特异性表达。采用差异表达倍数和差异表达百分比两种方法发现，两种分析模式下UVB中高表达的miRNA仅有35%重叠，但富集的信号通路有56.25%相同。UAB中高表达的miRNA仅有20%重叠，但信号通路有62.07%相同。进一步分析表明，miR-423可作为UVB的特征性miRNA，具有治疗肌肉相关疾病的潜力。miR-122-5p可作为UAB的特征性miRNA，可能有助于改善肝脏和大脑相关疾病。综上所述，这些结果丰富了对miRNA在母胎交流中的认识，为猪脐带血产品的开发和应用提供了参考。