Ma Jianfeng, Gan Mailin, Chen Jingyun, Chen Lei, Zhao Ye, Zhu Yan, Niu Lili, Zhang Shunhua, Jiang Yanzhi, Guo Zongyi, Wang Jinyong, Zhu Li, Shen Linyuan
Department of Animal Science, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China.
Farm Animal Genetic Resource Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.
Animals (Basel). 2022 Aug 17;12(16):2102. doi: 10.3390/ani12162102.
Intrauterine growth restriction (IUGR) is an important cause of newborn morbidity and mortality in mammals. Transfer RNA-derived small RNA (tsRNA) has become an emerging non-coding RNA in recent years. tsRNA and microRNAs (miRNAs) share similar mechanisms, which are involved in various biological processes. In this study, the pig was used as a model of IUGR, and the tsRNA and miRNA expression profile in the spleen was characterized by RNA sequencing. A total of 361 miRNAs and 620 tsRNAs were identified, of which 22 were differentially expressed miRNA (DEM) and 25 differentially expressed tsRNA (DET). tRF-5c were the primary tsRNA type making up more than 90%, and the most abundantly expressed tsRNAs are from tRNA-Gly-GCC. Functional enrichment analysis found that those DETs and DEMs have been implicated in the immune system process. Protein-protein interaction (PPI) network analysis revealed ssc-miR-370, ssc-miR-206, tiRNA-Ser-TGA-001 and tRF-Val-AAC-034 could be major regulators. TNF, TLR4, CD44, MAPK1 and STAT1 were predicted hub target genes. Those DETs and DEMs may regulate the T-cell receptor signaling pathway and Toll-like receptor signaling pathway to mediate the immunocompromise caused by IUGR. The results discussed in this article uncover the potential role of tsRNAs and miRNAs in IUGR porcine spleen.
宫内生长受限（IUGR）是哺乳动物新生儿发病和死亡的重要原因。近年来，转运RNA衍生的小RNA（tsRNA）已成为一种新兴的非编码RNA。tsRNA和微小RNA（miRNA）具有相似的机制，参与各种生物学过程。在本研究中，猪被用作IUGR模型，通过RNA测序对脾脏中的tsRNA和miRNA表达谱进行了表征。共鉴定出361个miRNA和620个tsRNA，其中22个是差异表达的miRNA（DEM），25个是差异表达的tsRNA（DET）。tRF-5c是主要的tsRNA类型，占比超过90%，表达量最高的tsRNAs来自tRNA-Gly-GCC。功能富集分析发现，这些DET和DEM与免疫系统过程有关。蛋白质-蛋白质相互作用（PPI）网络分析显示，ssc-miR-370、ssc-miR-206、tiRNA-Ser-TGA-001和tRF-Val-AAC-034可能是主要调节因子。TNF、TLR4、CD44、MAPK1和STAT1被预测为枢纽靶基因。这些DET和DEM可能通过调节T细胞受体信号通路和Toll样受体信号通路来介导IUGR引起的免疫功能低下。本文讨论的结果揭示了tsRNAs和miRNAs在IUGR猪脾脏中的潜在作用。
