Farm Animal Genetic Resource Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, 611130, China.
Key Laboratory of Livestock and Poultry Multi-Omics, Ministry of Agriculture and Rural Affairs, College of Animal and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
J Nanobiotechnology. 2023 Mar 9;21(1):83. doi: 10.1186/s12951-023-01837-y.
In this study, exosomes from cooked meat were extracted by ultra-high-speed centrifugation. Approximately 80% of exosome vesicles were within 20-200 nm. In addition, the surface biomarkers of isolated exosomes were evaluated using flow cytometry. Further studies showed the exosomal microRNA profiles were different among cooked porcine muscle, fat and liver. Cooked pork-derived exosomes were chronically administered to ICR mice by drinking for 80 days. The mice plasma levels of miR-1, miR-133a-3p, miR-206 and miR-99a were increased to varying degrees after drinking exosome enriched water. Furthermore, GTT and ITT results confirmed an abnormal glucose metabolism and insulin resistance in mice. Moreover, the lipid droplets were significantly increased in the mice liver. A transcriptome analysis performed with mice liver samples identified 446 differentially expressed genes (DEGs). Functional enrichment analysis found that DEGs were enriched in metabolic pathways. Overall, the results suggest that microRNAs derived form cooked pork may function as a critical regulator of metabolic disorder in mice.
在这项研究中，通过超速离心从熟肉中提取外泌体。大约 80%的外泌体囊泡在 20-200nm 之间。此外，使用流式细胞术评估了分离的外泌体表面生物标志物。进一步的研究表明，不同来源的熟猪肉、脂肪和肝脏中外泌体的 microRNA 图谱存在差异。用含外泌体的水通过饮用的方式对 ICR 小鼠进行为期 80 天的慢性给药。饮用富含外泌体的水后，小鼠血浆中 miR-1、miR-133a-3p、miR-206 和 miR-99a 的水平均不同程度地升高。此外，GTT 和 ITT 结果证实了小鼠的葡萄糖代谢异常和胰岛素抵抗。此外，小鼠肝脏中的脂质滴显著增加。对小鼠肝脏样本进行的转录组分析鉴定出 446 个差异表达基因（DEGs）。功能富集分析发现，DEGs 富集在代谢途径中。总的来说，这些结果表明，来源于熟猪肉的 microRNA 可能作为调节小鼠代谢紊乱的关键调控因子。
