Šušnjar Tomislav, Kuzmić Prusac Ivana, Švagelj Ivan, Jurišić Anđela, Šušnjar Tomislav, Jurišić Antonija, Jukić Miro, Pogorelić Zenon
Department of Pediatric Surgery, University Hospital of Split, 21000 Split, Croatia.
Department of Pathology, Forensic Medicine and Cytology, University Hospital of Split, 21000 Split, Croatia.
Animals (Basel). 2021 May 27;11(6):1569. doi: 10.3390/ani11061569.
The aim of this study was to compare consequences in single and triple testicular biopsy by biopty gun in pubertal rats using histological and immunohistochemical analysis.
Thirty-two Sprague-Dawley male rats were used as the experimental model. The rats were randomly divided into three study groups. The rats from the first group ( = 12) received a single-biopsy of upper pole of the left testis, while the rats from the second group ( = 10) received triple-biopsy of upper and lower poles and lateral surface of left testis. The third group ( = 10) was a control group. On the eightieth day after the biopsy in all rats bilateral orchiectomy and funiculectomy were performed to obtain testicular tissue and sperm for analysis. The consequences of the puncture were observed by pathohistology, immunohistochemistry and semen analysis.
The results of the study showed lower percentage of sperm count (14.5 mill/mL vs. 16 mill/mL, = 0.130), sperm motility (24.6% vs. 32.7%, > 0.05), abnormal sperm (30% vs. 27%, > 0.05), atrophic tubules (21% vs. 6%, < 0.001), volume (1.7 mL vs. 2.28 mL, < 0.01) and apoptotic index (1.56 vs. 1.19, = 0.650) in the testes with a triple-biopsy compared to the testes with a single-biopsy. Semen analysis showed a borderline significant difference between the group with triple-biopsy where sperm count was lower than it in the control group (14.5 mill/mL vs. 17.5 mill/mL, = 0.05). A single-biopsy has little effect on the testis, especially on overall fertility. A triple-biopsy showed higher degree of the testicular damage but without a significant impact on overall fertility. Semen analysis showed that single- and triple-biopsies did not have a significant effect on sperm count, motility and morphology.
Biopty gun procedure is a cheap, simple and reliable method for testicular biopsy in rats without a significant effect on sperm count, motility and morphology.
本研究的目的是通过组织学和免疫组织化学分析，比较青春期大鼠使用活检枪进行单次和三次睾丸活检的后果。
32只Sprague-Dawley雄性大鼠作为实验模型。大鼠被随机分为三个研究组。第一组（n = 12）的大鼠接受左侧睾丸上极的单次活检，而第二组（n = 10）的大鼠接受左侧睾丸上、下极及侧面的三次活检。第三组（n = 10）为对照组。在所有大鼠活检后第80天，进行双侧睾丸切除术和精索切除术，以获取睾丸组织和精子进行分析。通过病理组织学、免疫组织化学和精液分析观察穿刺的后果。
研究结果显示，与单次活检的睾丸相比，三次活检的睾丸精子计数百分比更低（14.5百万/毫升对16百万/毫升，P = 0.130）、精子活力更低（24.6%对32.7%，P>0.05）、异常精子比例更低（30%对27%，P>0.05）、萎缩小管比例更高（21%对6%，P<0.001）、体积更小（1.7毫升对2.28毫升，P<0.01）以及凋亡指数更高（1.56对1.19，P = 0.650）。精液分析显示，三次活检组的精子计数低于对照组（14.5百万/毫升对17.5百万/毫升，P = 0.05），差异接近显著。单次活检对睾丸影响较小，尤其是对总体生育能力。三次活检显示睾丸损伤程度更高，但对总体生育能力无显著影响。精液分析表明，单次和三次活检对精子计数、活力和形态均无显著影响。
活检枪操作是一种用于大鼠睾丸活检的廉价、简单且可靠的方法，对精子计数、活力和形态无显著影响。
