Miyaji Naomi, Akter Mst Arjina, Suzukamo Chizuko, Mehraj Hasan, Shindo Tomoe, Itabashi Takeru, Okazaki Keiichi, Shimizu Motoki, Kaji Makoto, Katsumata Masahiko, Dennis Elizabeth S, Fujimoto Ryo
Graduate School of Agricultural Science, Kobe University, Rokkodai, Nada-ku, Kobe 657-8501, Japan.
Iwate Biotechnology Research Center, Narita, Kitakami, Iwate 024-0003, Japan.
Plants (Basel). 2021 May 27;10(6):1082. doi: 10.3390/plants10061082.
In vegetables of L., f. sp. () or f. sp. () cause Fusarium yellows. A resistance gene against () has been identified, and deletion of this gene results in susceptibility (). In contrast, a resistance gene against has not been identified. Inoculation tests showed that lines resistant to were also resistant to , and lines susceptible to were susceptible to . However, prediction of disease resistance by a dominant DNA marker on (Bra012688m) was not associated with disease resistance of in some komatsuna lines using an inoculation test. QTL-seq using four F populations derived from susceptible and resistant lines showed one causative locus on chromosome A03, which covers . Comparison of the amino acid sequence of between susceptible and resistant alleles ( and ) showed that six amino acid differences were specific to susceptible lines. The presence and absence of is consistent with resistance in F populations. These results indicate that is essential for resistance, and changed amino acid sequences result in susceptibility to . This susceptible allele is termed , and a new DNA marker (focbr1-2m) for detection of the allele was developed.
在L.的蔬菜中，f. sp. （）或f. sp. （）会引发镰刀菌黄化病。已鉴定出一个针对（）的抗性基因，该基因的缺失会导致易感性（）。相比之下，尚未鉴定出针对的抗性基因。接种试验表明，对具有抗性的品系对也具有抗性，而对敏感的品系对也敏感。然而，在一些小松菜品系中，通过（Bra012688m）上的显性DNA标记预测抗病性与接种试验中对的抗病性无关。使用来自易感和抗性品系的四个F群体进行QTL-seq分析，在A03染色体上发现了一个致病位点，该位点覆盖了。比较易感和抗性等位基因（和）之间的氨基酸序列，发现六个氨基酸差异是易感品系特有的。在F群体中，的存在与否与抗性一致。这些结果表明，对于抗性至关重要，氨基酸序列的改变会导致对的易感性。这种易感等位基因被称为，并且开发了一种用于检测等位基因的新DNA标记（focbr1-2m）。
