The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Institute of Cereal and Oil Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050035, China.
Int J Mol Sci. 2021 May 26;22(11):5629. doi: 10.3390/ijms22115629.
Sharp eyespot, caused by necrotrophic fungus , is a serious fungal disease in wheat (). Certain wall-associated receptor kinases (WAK) mediate resistance to diseases caused by biotrophic/hemibiotrophic pathogens in several plant species. Yet, none of wheat genes with positive effect on the innate immune responses to has been reported. In this study, we identified a gene , located on chromosome 7D, and showed its positive regulatory role in the defense response to infection in wheat. RNA-seq and qRT-PCR analyses showed that transcript abundance was elevated in wheat after inoculation and the induction in the stem was the highest among the tested organs. Additionally, transcript levels were significantly elevated by pectin and chitin treatments. The knock-down of transcript impaired resistance to and repressed the expression of five pathogenesis-related genes in wheat. The green fluorescent protein signal distribution assays indicated that TaWAK7D localized on the plasma membrane in wheat protoplasts. Thus, TaWAK7D, which is induced by , pectin and chitin stimuli, positively participates in defense responses to through modulating the expression of several pathogenesis-related genes in wheat.
尖锐眼点病，由坏死型真菌引起，是小麦中的一种严重真菌病。某些壁相关受体激酶（WAK）在几种植物物种中介导对生物亲和/半生物亲和病原体引起的疾病的抗性。然而，尚未报道任何对小麦固有免疫反应具有积极影响的基因。在这项研究中，我们鉴定了一个基因，位于染色体 7D 上，并显示其在小麦对感染的防御反应中具有积极的调节作用。RNA-seq 和 qRT-PCR 分析表明，接种后小麦中基因的转录丰度升高，在测试的器官中，茎中的诱导最高。此外，果胶和几丁质处理显著提高了转录水平。基因转录本的敲低削弱了对的抗性，并抑制了小麦中五个病程相关基因的表达。绿色荧光蛋白信号分布测定表明，TaWAK7D 在小麦原生质体中定位于质膜上。因此，TaWAK7D 被诱导，果胶和几丁质刺激，通过调节小麦中几个病程相关基因的表达，积极参与对的防御反应。
