Guo Yong, Huang Yangrui, Li Jin, Wang Luwei, Yang Zhigang, Liu Jinyuan, Peng Xiao, Yan Wei, Qu Junle
College of Physics and Optoeletronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China.
Membranes (Basel). 2021 May 26;11(6):391. doi: 10.3390/membranes11060391.
We report a deep penetration microscopic imaging method with a non-diffracting Airy beam. The direct mapping of volume imaging in free space shows that the axial imaging range of the Airy beam is approximately 4 times that of the traditional Gaussian beam along the axial direction while maintaining a narrow lateral width. Benefiting from its non-diffracting property, the microscopic imaging with Airy beam illumination can acquire image structures through turbid medium and capture a volumetric image in a single frame. We demonstrate the penetration ability of the Airy microscopic imaging through a strongly scattering environment with 633 nm and 780 nm lasers. The performances of the volumetric imaging method were evaluated using HeLa cells and isolated mouse kidney tissue. The thick sample was scanned layer by layer in the Gaussian mode, however, in the Airy mode, the three-dimensional (3D) structure information was projected onto a two-dimensional (2D) image, which vastly increased the volume imaging speed. To show the characteristics of the Airy microscope, we performed dynamic volumetric imaging on the isolated mouse kidney tissue with two-photon.
我们报道了一种利用无衍射艾里光束的深度穿透显微成像方法。自由空间中体积成像的直接映射表明，艾里光束的轴向成像范围沿轴向约为传统高斯光束的4倍，同时保持较窄的横向宽度。受益于其无衍射特性，用艾里光束照明的显微成像可以通过混浊介质获取图像结构，并在单帧中捕获体积图像。我们通过633纳米和780纳米激光在强散射环境中展示了艾里显微成像的穿透能力。使用HeLa细胞和分离的小鼠肾脏组织评估了体积成像方法的性能。厚样品在高斯模式下逐层扫描，然而，在艾里模式下，三维（3D）结构信息被投影到二维（2D）图像上，这大大提高了体积成像速度。为了展示艾里显微镜的特性，我们用双光子对分离的小鼠肾脏组织进行了动态体积成像。
