利用超长消衍射光束对活体小鼠脑血管进行动态容积成像

Dynamic Volumetric Imaging of Mouse Cerebral Blood Vessels In Vivo with an Ultralong Anti-Diffracting Beam.

机构信息

State Key Laboratory of Radio Frequency Heterogeneous Integration (Shenzhen University), College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen University, Shenzhen 518060, China.

出版信息

Molecules. 2023 Jun 23;28(13):4936. doi: 10.3390/molecules28134936.

DOI:10.3390/molecules28134936

PMID:37446598

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10343797/

Abstract

Volumetric imaging of a mouse brain in vivo with one-photon and two-photon ultralong anti-diffracting (UAD) beam illumination was performed. The three-dimensional (3D) structure of blood vessels in the mouse brain were mapped to a two-dimensional (2D) image. The speed of volumetric imaging was significantly improved due to the long focal length of the UAD beam. Comparing one-photon and two-photon UAD beam volumetric imaging, we found that the imaging depth of two-photon volumetric imaging (80 μm) is better than that of one-photon volumetric imaging (60 μm), and the signal-to-background ratio (SBR) of two-photon volumetric imaging is two times that of one-photon volumetric imaging. Therefore, we used two-photon UAD volumetric imaging to perform dynamic volumetric imaging of mouse brain blood vessels in vivo, and obtained the blood flow velocity.

摘要

利用单光子和双光子超远距无衍射（UAD）光束照明对活体小鼠大脑进行容积成像。将小鼠大脑血管的三维（3D）结构映射到二维（2D）图像上。由于 UAD 光束的长焦距，容积成像的速度得到了显著提高。通过比较单光子和双光子 UAD 光束容积成像，我们发现双光子容积成像的成像深度（80μm）优于单光子容积成像（60μm），并且双光子容积成像的信噪比（SBR）是单光子容积成像的两倍。因此，我们使用双光子 UAD 容积成像对活体小鼠大脑血管进行动态容积成像，并获得了血流速度。