Opt Lett. 2019 Jan 15;44(2):391-394. doi: 10.1364/OL.44.000391.
We demonstrate a volumetric two-photon microscopy (TPM) using the non-diffracting Airy beam as illumination. Direct mapping of the imaging trajectory shows that the Airy beam extends the axial imaging range around six times longer than a traditional Gaussian beam does along the propagation direction, while maintaining a comparable lateral width. Benefiting from its non-diffracting nature, the TPM with Airy beam illumination is able not only to capture a volumetric image within a single frame, but also to acquire image structures behind a strongly scattered medium. The volumetric specimen is mapped layer by layer under Gaussian mode, while the three-dimensional structure is projected to a single two-dimensional image under Airy mode, leading to a significantly increased acquisition speed. The performance of the TPM is evaluated employing a phantom of agarose gel imbedding fluorescent beads as well as a mouse brain slice. Finally, we showcase the penetration ability of the developed Airy TPM by imaging through a scattering environment.
我们展示了一种使用无衍射艾里光束作为照明的体积双光子显微镜（TPM）。成像轨迹的直接映射表明，与传统的高斯光束相比，艾里光束在传播方向上沿轴向将成像范围扩展了大约六倍，同时保持了相当的横向宽度。受益于其无衍射特性，使用艾里光束照明的 TPM 不仅能够在单个帧内捕获体积图像，还能够获取强散射介质后面的图像结构。体积标本在高斯模式下逐层映射，而三维结构在艾里模式下投影到单个二维图像上，从而显著提高了采集速度。使用琼脂糖凝胶嵌入荧光珠的幻影以及小鼠脑切片对 TPM 的性能进行了评估。最后，我们通过对散射环境进行成像展示了所开发的艾里 TPM 的穿透能力。
