黎巴嫩存在西尼罗河病毒循环。
Evidence of West Nile Virus Circulation in Lebanon.
机构信息
Laboratory of Immunology and Vector-Borne Diseases, Faculty of Public Health, Lebanese University, Fanar 6573/14, Lebanon.
Arbovirus Laboratories, Wadsworth Center, New York State Department of Health, Albany, NY 12159, USA.
出版信息
Viruses. 2021 May 26;13(6):994. doi: 10.3390/v13060994.
West Nile virus (WNV) has never been reported from Lebanon. Yet, this country is located on the flyway of migratory birds in the Middle East region. Serological screening was conducted to assess the potential circulation of this virus. Human, horse, and chicken sera were collected from the Bekaa and North districts. Specific IgG and IgY were first screened by ELISA. Then, positive samples were confirmed by plaque reduction neutralization test (PRNT). Besides this, adult mosquitoes were collected and tested for the presence of WNV RNA using conventional RT-PCR. Sera screening revealed a seroprevalence rate reaching 1.86% among humans and 2.47% among horses. Cross-reactions revealed by ELISA suggested the circulation of flaviviruses other than WNV. None of the tested mosquitoes was positive for WNV. The observed results constitute strong evidence of local exposure of the Lebanese population to this virus and the first report of equine WNV in Lebanon.
西尼罗河病毒（WNV）从未在黎巴嫩报告过。然而，这个国家位于中东地区候鸟迁徙路线上。进行血清学筛查以评估该病毒的潜在传播。从贝卡和北部地区收集了人类、马和鸡的血清。首先通过 ELISA 筛选特异性 IgG 和 IgY。然后，通过蚀斑减少中和试验（PRNT）确认阳性样本。此外，使用常规 RT-PCR 收集成年蚊子并检测WNV RNA 的存在。血清筛查显示人类的血清阳性率达到 1.86%，马的血清阳性率达到 2.47%。ELISA 显示的交叉反应表明除 WNV 以外的黄病毒的传播。没有检测到蚊子对 WNV 呈阳性。观察到的结果有力地证明了黎巴嫩人群接触该病毒的局部暴露，以及黎巴嫩首次报告马 WNV。
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