Cranford Hannah M, Taylor Marissa, Browne Andrew Springer, Alt David P, Anderson Tammy, Hamond Camila, Hornsby Richard L, LeCount Karen, Schlater Linda, Stuber Tod, De Wilde Leah, Burke-France Valicia J, Ellis Esther M, Nally Jarlath E, Bradford Bethany
Virgin Islands Department of Health, Epidemiology Division, Virgin Islands, Christiansted, VI 00820, USA.
Domestic Animal Health Analytics Team, Animal and Plant Health Inspection Service (APHIS), United States Department of Agriculture, Fort Colins, CO 80526, USA.
Trop Med Infect Dis. 2021 May 24;6(2):85. doi: 10.3390/tropicalmed6020085.
From 2019-2020, the Virgin Islands Department of Health (VIDOH) investigated potential animal reservoirs of spp., the pathogenic bacteria that cause leptospirosis. We examined exposure and carriage in livestock on the island of St. Croix, United States Virgin Islands (USVI). We utilized the microscopic agglutination test (MAT) to evaluate the sera, and the fluorescent antibody test (FAT), real time polymerase chain reaction (rt-PCR), and bacterial culture to evaluate urine specimens from livestock (n = 126): 28 cattle, 19 goats, 46 pigs, and 33 sheep. Seropositivity was 37.6% (47/125) with agglutinating antibodies to the following serogroups identified: Australis, Djasiman, Icterohaemorrhagiae, Ballum, Sejroe, Cynopteri, Autumnalis, Hebdomadis, Pomona, Canicola, Grippotyphosa, and Pyrogenes. Urine from 4 animals (4.0%, 4/101) was positive by rt-PCR for lipL32: 2 sheep, 1 goat, and 1 bull. Sequencing of secY amplicons identified . in 1 sheep and 1 bull. Livestock in USVI harbor pathogenic bacteria and could play a role in the zoonotic cycle of leptospirosis.
从2019年至2020年，美属维尔京群岛卫生部（VIDOH）对引起钩端螺旋体病的致病性细菌——问号钩端螺旋体的潜在动物宿主进行了调查。我们在美国维尔京群岛圣克罗伊岛的家畜中检测了问号钩端螺旋体的暴露情况和携带情况。我们利用显微镜凝集试验（MAT）评估血清，并利用荧光抗体试验（FAT）、实时聚合酶链反应（rt-PCR）和细菌培养来评估家畜（n = 126）的尿液样本：28头牛、19只山羊、46头猪和33只绵羊。血清阳性率为37.6%（47/125），鉴定出针对以下血清群的凝集抗体：澳洲群、爪哇群、出血性黄疸群、拜伦群、七日热群、犬蝠群、秋季群、七日群、波摩那群、犬群、致热群和热解群。4只动物（4.0%，4/101）的尿液经rt-PCR检测lipL32呈阳性：2只绵羊、1只山羊和1头公牛。secY扩增子测序在1只绵羊和1头公牛中鉴定出问号钩端螺旋体。美属维尔京群岛的家畜携带致病性问号钩端螺旋体细菌，可能在钩端螺旋体病的人畜共患病循环中发挥作用。
