Haji Hajikolaei Mohammad Rahim, Rezaei Sareh, Ghadrdan Mashhadi Ali Reza, Ghorbanpoor Masoud
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
Sci Rep. 2022 Feb 9;12(1):2148. doi: 10.1038/s41598-022-05767-x.
Leptospirosis is a common global zoonotic disease of man and all farm animals. Although most leptospiral infections in sheep and goats are asymptomatic, they may play a role in the epidemiology of the disease by the spread of Leptospira through the urine. This study was carried out to evaluate the role of sheep and goats in the epidemiology of leptospirosis. Blood and urine samples were taken from 210 goats and 246 sheep. To detect antibodies, sera samples were tested with 8 live serovars of L. interrogans (Hardjo, Pomona, Grippotyphosa, Canicola, Ballum, Icterhemorrhagiae, Tarasovi, and Australis) by MAT. Then, urine samples were tested by Nested PCR targeting 16S rRNA gene for detection of pathogenic Leptospira. Results of MAT showed that 10.95% of goats and 8.53% of sheep had antibodies against at least one examined serovars. In both species, the highest reacting was L. i. Pomona with a rate of 68.18% and 56% in sheep and goats, respectively. Moreover, in PCR, 2 (0.95%) urine samples of goat and 12 (4.87%) urine samples of sheep were positive. All of the MAT positive studied animals were PCR negative and, statistical analysis showed that there was no relationship and agreement between the results of PCR and MAT in sheep (kappa = - 0.07, p > 0.05) and goats (kappa = - 0.02, p > 0.05). Finally, it is concluded that sheep and goats can excrete L. interrogans in the urine and thus transmit them to other animals and humans.
钩端螺旋体病是一种常见的全球性人畜共患病，可感染人类和所有农场动物。虽然绵羊和山羊的大多数钩端螺旋体感染没有症状，但它们可能通过尿液传播钩端螺旋体，在该病的流行病学中发挥作用。本研究旨在评估绵羊和山羊在钩端螺旋体病流行病学中的作用。采集了210只山羊和246只绵羊的血液和尿液样本。为检测抗体，通过显微镜凝集试验（MAT）用8种问号钩端螺旋体活血清型（哈德乔型、波摩那型、致热型、犬型、拜伦型、出血黄疸型、塔拉索夫型和澳洲型）检测血清样本。然后，通过针对16S rRNA基因的巢式聚合酶链反应（Nested PCR）检测尿液样本，以检测致病性钩端螺旋体。MAT结果显示，10.95%的山羊和8.53%的绵羊至少对一种检测的血清型有抗体。在这两个物种中，反应最强的是波摩那型问号钩端螺旋体，在绵羊和山羊中的反应率分别为68.18%和56%。此外，在PCR检测中，2份（0.95%）山羊尿液样本和12份（4.87%）绵羊尿液样本呈阳性。所有MAT阳性的受试动物PCR检测均为阴性，统计分析表明，绵羊（kappa = -0.07，p > 0.05）和山羊（kappa = -0.02，p > 0.05）的PCR和MAT结果之间没有相关性和一致性。最后得出结论，绵羊和山羊可通过尿液排出问号钩端螺旋体，从而将其传播给其他动物和人类。
