Infectious Bacterial Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, IA, USA.
Sci Rep. 2020 Jun 15;10(1):9620. doi: 10.1038/s41598-020-66526-4.
The causative agent of leptospirosis includes multiple serovars and species of pathogenic leptospires that are excreted via urine from reservoir hosts of infection. Primary isolation takes weeks to months, and is limited to semi-solid media at 28-30 °C. Here we present an alternative media formulation, HAN, compared to commercially available EMJH and the more specialized T80/40/LH media formulations, in semi-solid and liquid compositions, for the primary isolation of two diverse species and serovars of pathogenic leptospires directly from host kidney tissue. All three media types supported the isolation and propagation of L. interrogans serovar Copenhageni strain IC:20:001 in semi-solid media at 29 °C. However, only HAN and T80/40/LH supported the growth of L. borgpetersenii serovar Hardjo strain HB15B203 at 29 °C. In addition, HAN supported primary isolation at 37 °C. Both T80/40/LH and HAN supported primary isolation of strain IC:20:001 in liquid media at 29 °C but only HAN supported growth of strain HB15B203 in liquid media, at both 29 and 37 °C. HAN media supports the primary isolation of fastidious pathogenic leptospires directly from infected host tissue at either 29 or 37 °C: this formulation represents a more defined media for the continued optimization of growth factors required to support the primary isolation of the large and diverse range of species and serovars within the genus Leptospira circulating within domestic and wild animal populations.
钩端螺旋体病的病原体包括多种血清型和致病性钩端螺旋体，这些病原体通过感染宿主的尿液排出。初次分离需要数周至数月的时间，且仅局限于 28-30°C 的半固体培养基。在这里，我们提出了一种替代培养基配方 HAN，与市售的 EMJH 以及更专业的 T80/40/LH 培养基配方相比，在半固体和液体配方中，用于直接从宿主肾脏组织中分离两种不同种和血清型的致病性钩端螺旋体。三种培养基类型都支持 29°C 半固体培养基中 L. interrogans 血清型 Copenhageni 菌株 IC:20:001 的分离和繁殖。然而，只有 HAN 和 T80/40/LH 支持 L. borgpetersenii 血清型 Hardjo 菌株 HB15B203 在 29°C 的生长。此外，HAN 支持 37°C 的初次分离。T80/40/LH 和 HAN 均支持 29°C 下液体培养基中菌株 IC:20:001 的初次分离，但只有 HAN 支持菌株 HB15B203 在液体培养基中的生长，无论是在 29°C 还是 37°C。HAN 培养基支持在 29 或 37°C 下直接从感染宿主组织中分离出挑剔的致病性钩端螺旋体：这种配方代表了一种更明确的培养基，可以进一步优化支持属内大量不同种和血清型的致病性钩端螺旋体的初次分离所需的生长因子。这些种和血清型在家庭和野生动物种群中循环。
