MINTOTA Research Group, Department of Analytical Chemistry, University of Valencia, Dr. Moliner 50, 46100, Burjassot, Valencia, Spain.
MINTOTA Research Group, Department of Analytical Chemistry, University of Valencia, Dr. Moliner 50, 46100, Burjassot, Valencia, Spain.
Talanta. 2021 Sep 1;232:122406. doi: 10.1016/j.talanta.2021.122406. Epub 2021 Apr 20.
Scopolamine (SCP) is often involved in sexual assaults and robberies, particularly in recreational environments. Therefore, analytical tools are required for the analysis of this compound amenable for the field. In this work, a sensor for SCP is described based on the entrapment of KMnO into polydimethylsiloxane (PDMS). The possibility of using KMnO in combination with the reagent 1,2-naphtoquinone-4-sulfonate (NQS) giving a double sensor acting as a bicolorimetric device is also demonstrated. In contact with the sample, the PDMS composite delivers MnO, which reacts rapidly with SCP under basic conditions causing a change of the color of the solution that can be related to the concentration of drug using both, absorbances and color coordinates, while the NQS part of the sensor remains unchanged. After an exposure time to the sensors of 10 min, satisfactory linearity was obtained for concentrations of SCP up to 865 μg/mL, being the limit of detection (LOD) 108 μg/mL. A method using a portable nano liquid chromatograph with detection at 255 nm has been also developed; in this case the LOD was 100 μg/mL and the working linear interval was 250-2000 μg/mL. The precision, expressed as relative standard deviation (RDS), was ≤8% for both methods. Different beverages (cola, cola-whisky, tonic water-vodka, red wine and green tea) were assayed. The potential of the two proposed approaches for on-site tests is discussed.
莨菪碱（SCP）常用于性侵犯和抢劫案件中，尤其是在娱乐环境中。因此，需要有分析工具来对这种化合物进行分析，以便于现场使用。在这项工作中，描述了一种基于 KMnO 捕获到聚二甲基硅氧烷（PDMS）中的 SCP 传感器。还证明了 KMnO 与试剂 1,2-萘醌-4-磺酸盐（NQS）结合使用的可能性，该试剂可作为双色比色装置的双传感器。与样品接触时，PDMS 复合材料会释放 MnO，MnO 在碱性条件下与 SCP 迅速反应，导致溶液颜色发生变化，可通过吸光度和颜色坐标来对应药物浓度，而传感器的 NQS 部分保持不变。将传感器暴露于样品 10 分钟后，对于 SCP 的浓度，获得了高达 865μg/mL 的满意线性度，检测限（LOD）为 108μg/mL。还开发了一种使用带有 255nm 检测的便携式纳升级液相色谱法的方法；在这种情况下，LOD 为 100μg/mL，工作线性区间为 250-2000μg/mL。两种方法的精密度（表示为相对标准偏差（RDS））均≤8%。对不同的饮料（可乐、可乐威士忌、汤力水伏特加、红酒和绿茶）进行了测定。讨论了这两种方法在现场测试中的应用潜力。
