MINTOTA Research Group, Departament de Química Analítica, Universitat de València, Dr. Moliner 50, 46100 Burjassot, Spain.
Molecules. 2021 Nov 5;26(21):6713. doi: 10.3390/molecules26216713.
Due to their simplicity, speed and low cost, chemical spot tests are increasingly demanded for the presumptive identification of illicit drugs in a variety of contexts such as point-of-care assistance or prosecution of drug trafficking. However, most of the colorimetric reactions used in these tests are, at best, drug class selective. Therefore, the development of tests based on chemical reactions with improved discrimination power is of great interest. In this work, we propose a new colorimetric assay for amphetamine (AMP) based on its reaction with solutions of alkaline gold bromide to form an insoluble yellow-orange derivative. The resulting suspensions are then filtered onto nylon membranes and the precipitate collected is used for the visual identification of AMP. The measurement of the absorbance of the membranes by diffuse reflectance spectroscopy also allows the quantification of AMP in a simple and rapid way, as demonstrated for different synthetic and drug street samples. On the basis of the results obtained, it was concluded that the proposed procedure is highly selective towards AMP, as this compound could be easily differentiated from other common drugs such as methamphetamine (MET), ephedrine (EPH), scopolamine (SCP) and cocaine (COC).
由于其简单、快速和低成本，化学斑点测试越来越多地被要求用于在各种情况下对非法药物进行初步鉴定，例如在护理点提供帮助或起诉贩毒。然而，这些测试中使用的大多数比色反应充其量只是药物类别选择性的。因此，开发基于具有改进分辨能力的化学反应的测试具有很大的意义。在这项工作中，我们提出了一种基于与碱性溴化金溶液反应形成不溶性黄橙色衍生物的新的安非他命（AMP）比色测定法。然后将所得悬浮液过滤到尼龙膜上，并收集沉淀物用于 AMP 的目视鉴定。通过漫反射光谱法测量膜的吸光度也可以简单快速地定量 AMP，这已在不同的合成和药物街头样品中得到证明。根据获得的结果，可以得出结论，所提出的程序对 AMP 具有高度选择性，因为可以很容易地将该化合物与其他常见药物如甲基苯丙胺（MET）、麻黄碱（EPH）、莨菪碱（SCP）和可卡因（COC）区分开来。
