MINTOTA Research Group, Departament de Química Analítica, Facultat de Química, Universitat de València, Dr. Moliner 50, 46100 Burjassot, Spain.
Molecules. 2023 Jan 30;28(3):1303. doi: 10.3390/molecules28031303.
A colorimetric method for the estimation of the total content of cannabinoids in cannabis samples is proposed. The assay is based on the reaction of these compounds with the reagent Fast Blue B (FBB), which has been immobilized into polydimethylsiloxane (PDMS). The reaction and detection conditions have been established according to the results obtained for the individual cannabinoids Δ-tetrahydrocannabidiol (THC), cannabidiol (CBD), and cannabinol (CBN), as well as for ethanolic extracts obtained from cannabis samples after ultrasonication. In contact with the extract and under basic conditions, the reagent diffuses from the PDMS device, producing a red-brown solution. The absorbances measured at 500 nm after only 1 min of exposure to the FBB/PDMS composites led to responses proportional to the amounts of the cannabinoids in the reaction media. Those absorbances have been then transformed in total cannabinoid content using CBD as a reference compound. The potential utility of the proposed conditions has been tested by analyzing different cannabis samples. The selectivity towards other plants and drugs has been also evaluated. The present method is proposed as a simple and rapid alternative to chromatographic methods for the estimation of the total content of cannabinoids.
提出了一种比色法来估算大麻样品中总大麻素含量。该测定法基于这些化合物与试剂 Fast Blue B（FBB）的反应，FBB 已被固定到聚二甲基硅氧烷（PDMS）中。根据对个别大麻素 Δ-四氢大麻酚（THC）、大麻二酚（CBD）和大麻醇（CBN）以及超声处理后的大麻样品的乙醇提取物的反应和检测条件的结果进行了建立。在与提取物接触并在碱性条件下，试剂从 PDMS 装置中扩散出来，产生红棕色溶液。在暴露于 FBB/PDMS 复合材料仅 1 分钟后测量的 500nm 处的吸光度导致与反应介质中大麻素含量成正比的响应。然后使用 CBD 作为参考化合物，将这些吸光度转化为总大麻素含量。通过分析不同的大麻样品，测试了所提出条件的潜在用途。还评估了对其他植物和药物的选择性。该方法被提议作为一种简单快速的替代色谱方法，用于估算大麻素的总量。
