CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, Gansu, 730000, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, Gansu, 730000, China.
Talanta. 2021 Sep 1;232:122459. doi: 10.1016/j.talanta.2021.122459. Epub 2021 May 8.
The nickel organic framework capped with polyvinylpyrrolidone was prepared and synergistically immobilized onto porous silica surface as the mixed-mode stationary phase for high-performance liquid chromatography. Here, polyvinylpyrrolidone firstly was chosen as functional molecules to change morphology and size of the metal organic framework. The silica microspheres were then modified by them via a simple bonding method rather than in-situ growth method with the aid of electrostatic interaction commonly used before. The stationary phase showed flexible selectivity for separation of both hydrophilic and hydrophobic compounds, especially for hydrophilic compounds such as carbohydrates, alkaloids and sulfonamides etc. The chromatographic behaviors were evaluated by investigating various factors, and a typical mixed-mode retention feature of the column was observed. The composites could be prepared repetitively, and relative standard deviations of retention time of objective compounds among different batches were less than 1.75%. It also showed excellent chromatographic reproducibility, stability and potentiality for application in real samples. In short, the composites can be used for a feasible option for analysis of multiple compounds as the mixed-mode stationary phase and it provides a general approach for preparing MOFs-based composites by changing morphology and size of MOFs.
采用聚乙烯吡咯烷酮封端的镍有机框架，并协同固定在多孔硅胶表面上，作为高效液相色谱的混合模式固定相。在这里，聚乙烯吡咯烷酮首先被选择作为功能分子来改变金属有机框架的形态和尺寸。然后，通过一种简单的键合方法而不是以前常用的原位生长方法，将硅胶微球与它们进行修饰，借助于静电相互作用。固定相对亲水和疏水化合物的分离表现出灵活的选择性，特别是对亲水化合物，如碳水化合物、生物碱和磺胺类药物等。通过研究各种因素来评估色谱行为，并观察到柱的典型混合模式保留特征。该复合材料可重复制备，不同批次目标化合物的保留时间的相对标准偏差小于 1.75%。它还表现出出色的色谱重现性、稳定性和在实际样品分析中的应用潜力。总之，该复合材料可用作分析多种化合物的可行选择，作为混合模式固定相，为通过改变金属有机框架的形态和尺寸来制备基于金属有机框架的复合材料提供了一种通用方法。
