Raabová Hedvika, Erben Jakub, Chvojka Jiří, Solich Petr, Švec František, Šatínský Dalibor
Charles University, Faculty of Pharmacy in Hradec Králové, Department of Analytical Chemistry, Akademika Heyrovského 1203, 50005, Hradec Králové, Czech Republic.
The Technical University of Liberec, Faculty of Textile Engineering, Department of Nonwovens and Nanofibrous Materials, Studentská 1402/2, 46001, Liberec 1, Czech Republic.
Talanta. 2021 Sep 1;232:122470. doi: 10.1016/j.talanta.2021.122470. Epub 2021 Apr 30.
Effect of physicochemical properties including dissociation constant (pKa) and partition coefficient (log P) of the compounds on their extraction efficiency in sample preparation using fibrous polymer sorbents has been demonstrated. Poly-ε-caprolactone as meltblown/electrospun composite fibers, and polypropylene, polyethylene, poly(3-hydroxybutyrate), poly(lactic acid), and polyamide 6 in the meltblown fiber format were used as sorbents in solid-phase extraction. In addition, the polycaprolactone fibers were coated with dopamine, dopamine combined with heparin, and tannin, respectively, to modify their extraction properties. These fibers that were not yet used for extractions and the unique combination of sorbents and analytes significantly extends the scope of nanofibrous extraction. The extraction efficiency was determined using model pharmaceuticals including acetylsalicylic acid, moxonidine, metoprolol, propranolol, propafenone, diltiazem, atorvastatin, and amiodarone. These model compounds displayed the widest differences in both pKa and log P values. The extraction efficiency of some of the fibers reached 96.64%. Coating of polycaprolactone fibers with dopamine significantly improved extraction efficiency of slightly retained metoprolol while moxonidine was not retained on any sorbent. The fibrous sorbents were also tested for extraction of pharmaceuticals in bovine serum albumin and human serum, respectively, to demonstrate their capability to extract them from a complex protein-containing matrix. The clean-up efficiency of our fibers was compared with that of a commercial restricted access media (RAM) C-18 alkyl-diol silica column. Our technique is in accordance with the requirements of modern sample preparation techniques.
已证明化合物的物理化学性质（包括解离常数（pKa）和分配系数（log P））对其在使用纤维状聚合物吸附剂的样品制备中的萃取效率的影响。聚ε-己内酯作为熔喷/电纺复合纤维，以及熔喷纤维形式的聚丙烯、聚乙烯、聚（3-羟基丁酸酯）、聚乳酸和聚酰胺6被用作固相萃取中的吸附剂。此外，聚己内酯纤维分别用多巴胺、多巴胺与肝素的组合以及单宁进行涂层，以改变其萃取性能。这些尚未用于萃取的纤维以及吸附剂和分析物的独特组合显著扩展了纳米纤维萃取的范围。使用包括乙酰水杨酸、莫索尼定、美托洛尔、普萘洛尔、普罗帕酮、地尔硫卓、阿托伐他汀和胺碘酮在内的模型药物测定萃取效率。这些模型化合物在pKa和log P值方面显示出最大的差异。一些纤维的萃取效率达到了96.64%。用多巴胺涂覆聚己内酯纤维显著提高了轻微保留的美托洛尔的萃取效率，而莫索尼定未保留在任何吸附剂上。还分别测试了纤维状吸附剂在牛血清白蛋白和人血清中对药物的萃取，以证明它们从含复杂蛋白质的基质中萃取药物的能力。将我们的纤维的净化效率与商业限进介质（RAM）C-18烷基二醇硅胶柱的净化效率进行了比较。我们的技术符合现代样品制备技术的要求。
