Department of Chemistry, Faculty of Arts & Sciences, University of Petra, P. O. Box 961343, Queen Alia Airport Street, Amman, 11196, Jordan.
Department of Pharmaceutics & Pharmaceutical Technology, Faculty of Pharmacy, University of Petra, P. O. Box 961343, Queen Alia Airport Street, Amman, 11196, Jordan.
Bioanalysis. 2024;16(16):883-894. doi: 10.1080/17576180.2024.2377908. Epub 2024 Aug 8.
The aim was to evaluate drug-plasma binding (DPB).by employing Hollow Fiber-in-Syringe Equilibrium Sampling Through Supported Liquid Membrane (HFiS ESTSLM) and RP-HPLC analysis. HFiS ESTSLM and RP-HPLC were used to evaluate DPB of three weak basic drugs (Metoprolol, Diphenhydramine, and Sildenafil) with differing hydrophilicity and binding ability to blood plasma. The results exhibited an increasing drug-dependent magnitude of DPB for the three model drugs. This trend of DPB confirmed that HFiS ESTSLM has the required sensitivity for determining DPB of the drugs. The DPB was drug concentration-dependent within the tested drug concentration range, especially at high concentration. HFiS ESTSLM and RP-HPLC offered a simple, easy and cost-effective procedure to evaluate DPB of these basic drugs.
目的是通过采用中空纤维在注射器中的平衡取样透过支撑液膜（HFiS ESTSLM）和反相高效液相色谱（RP-HPLC）分析来评估药物-血浆结合（DPB）。HFiS ESTSLM 和 RP-HPLC 用于评估三种弱碱性药物（美托洛尔、苯海拉明和西地那非）的 DPB，它们的亲水性和与血浆结合的能力不同。结果显示，三种模型药物的 DPB 呈药物依赖性增加的趋势。这种 DPB 趋势证实了 HFiS ESTSLM 具有确定药物 DPB 的所需灵敏度。DPB 与测试药物浓度范围内的药物浓度呈依赖性，尤其是在高浓度下。HFiS ESTSLM 和 RP-HPLC 提供了一种简单、易用且具有成本效益的方法来评估这些碱性药物的 DPB。
