Beijing Research Center for Information Technology in Agriculture, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China; College of Landscape and Ecological Engineering, Hebei University of Engineering, Handan, 056038, China.
Beijing Research Center for Information Technology in Agriculture, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.
Talanta. 2021 Sep 1;232:122477. doi: 10.1016/j.talanta.2021.122477. Epub 2021 Apr 30.
Methyl jasmonate (MeJA) is an endogenous plant hormone, which plays an important role in agriculture production. A novel probe-free electrochemical immunosensor was fabricated for detecting of MeJA. Fc, carboxylated graphene (COOH-GR) and carboxylated multi-walled carbon nanotubes (COOH-MWNT) composite was formed and used to fabricate screen-printed electrode (SPE). Fc was used as the electronic medium. COOH-GR and COOH-MWNT were used to improve the conductivity and catalytic activity of the sensor and to immobilize the MeJA antibody. Thus, the immunosensor can be used to detect MeJA without external redox probe solution. The designed sensor can detect MeJA in a wide range of 100 fM-100 μM, and its detection limit is as low as 31.26 fM (S/N = 3). The as-prepared probe-free immunosensor is simple, low cost, and does not need redox probe solutions for measurements, which shows great promise for future application in precision agriculture.
茉莉酸甲酯（MeJA）是一种内源性植物激素，在农业生产中起着重要作用。本文制备了一种新型无探针电化学免疫传感器用于检测 MeJA。Fc、羧基化石墨烯（COOH-GR）和羧基化多壁碳纳米管（COOH-MWNT）的复合材料被形成并用于制备丝网印刷电极（SPE）。Fc 被用作电子介质。COOH-GR 和 COOH-MWNT 被用于提高传感器的导电性和催化活性，并固定 MeJA 抗体。因此，该传感器可以在没有外部氧化还原探针溶液的情况下用于检测 MeJA。设计的传感器可以在 100 fM-100 μM 的宽范围内检测 MeJA，检测限低至 31.26 fM（S/N = 3）。所制备的无探针免疫传感器简单、低成本，且不需要氧化还原探针溶液进行测量，在精准农业的未来应用中具有广阔的前景。
